{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1303704000},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"13676.zip","Size":53937613,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"13676.tif","Size":54000000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"13676.jpg","Size":44308,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/3004\/"}],"PUBLISHED":["J Cell Biol. 2010. 191: 211-223."],"Contributors":["Xin Xiong","Xin Wang","Ronny Ewanek","Pavan Bhat","Aaron DiAntonio","Catherine A. Collins"],"PUBMED":["20921142"]},"BIOLOGICALPROCESS":[{"onto_name":"response to wounding","onto_id":"GO:0009611"},{"onto_name":"JNK cascade","onto_id":"GO:0007254"},{"onto_name":"negative regulation of JNK cascade","onto_id":"GO:0046329"},{"onto_name":"central nervous system development","onto_id":"GO:0007417"},{"onto_name":"negative regulation of mRNA 3'-end processing","onto_id":"GO:0031441"}],"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"The JNK phosphatase puckered, whose expression can be reported via a lacZ enhancer trap, is expressed in very low levels in uninjured neurons; however, nerve crush induces a dramatic increase in puc-lacZ expression in injured motoneurons. This image stack shows puc-lacZ (green) and neuronal nuclei (red) in a third instar larva injured at site 2 (as defined in Fig 1C). Wandering third instar larvae were dissected in PBS and fixed in either 4% paraformaldehyde in PBS or Bouin\u2019s fixative for 15\u201330 min. Primary antibodies used were: rat anti-elaV (7E8A10, Developmental Studies Hybridoma Bank) and mouse anti-lacZ (40-1a, DSHB). Secondary antibodies were Alexa 488 anti-mouse and Cy3 anti-rat. Confocal images were collected at room temperature on a spinning-disk confocal system (PerkinElmer) consisting of a scanner (Nipkow CSU10; Yokogawa) and an electron microscopy charge-coupled device camera (C9100-50; Hamamatsu Photonics) mounted on an inverted microscope (Axio Observer; Carl Zeiss, Inc.) with 25× 0.8 NA multi and 40× 1.3 NA, 63× 1.5 NA, and 100× 1.46 NA oil objectives. Similar settings were used to collect all compared genotypes. All imaging and quantification were conducted with Volocity software (PerkinElmer). This image stack corresponds to Fig 1E2 in J Cell Biol. 2010. 191: 211-223. Images in Fig 1 include  CIL#s 13676, 13677, 13678, 13679."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"nucleus","onto_id":"GO:0005634"},"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"7399","MOLECULARFUNCTION":[{"onto_name":"JUN kinase phosphatase activity","onto_id":"GO:0008579"},{"onto_name":"RNA binding","onto_id":"GO:0003723"}],"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"spinning disk confocal microscopy","onto_id":"FBbi:00000253"},"DIMENSION":[{"Space":{"Image_size":962,"Pixel_size":{"unit":"microns","value":0.5869},"axis":"X"}},{"Space":{"Image_size":1000,"Pixel_size":{"unit":"microns","value":0.5869},"axis":"Y"}},{"Space":{"Image_size":14,"Pixel_size":{"unit":"microns","value":0.8},"axis":"Z"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Drosophila melanogaster","onto_id":"NCBITaxon:7227"},"PREPARATION":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"VISUALIZATIONMETHODS":[{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},{"onto_name":"beta-galactosidase","onto_id":"FBbi:00000077"},{"onto_name":"Alexa Fluor 488","onto_id":"FBbi:00000440"},{"onto_name":"Cy3","onto_id":"FBbi:00000449"}],"CELLTYPE":{"onto_name":"motor neuron","onto_id":"CL:0000100"}}},"Citation":{"Title":"Xin Xiong, Xin Wang, Ronny Ewanek, Pavan Bhat, Aaron DiAntonio, Catherine A. Collins (2011) CIL:13676, Drosophila melanogaster, motor neuron. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13676","DOI":"doi:10.7295\/W9CIL13676"}}}