{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1303099200},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"13378.tif","Size":46400000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"13378.zip","Size":46330305,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"13378.jpg","Size":19000,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"Journal of Cell Biology","Href":"http:\/\/jcb.rupress.org\/content\/179\/2\/187.long"}],"PUBLISHED":["JCB vol. 179 no. 2 187-197"],"Contributors":["Iain M. Porter","Sarah E. McClelland","Guennadi A. Khoudoli","Christopher J. Hunter","Jens S. Andersen","Andrew D. McAinsh","J. Julian Blow","Jason R. Swedlow"],"PUBMED":["17938248"]},"BIOLOGICALPROCESS":[{"onto_name":"mitosis","onto_id":"GO:0007067"},{"free_text":"Bod1 SiRNA"},{"onto_name":"chromosome organization","onto_id":"GO:0051276"}],"PROCESSINGHISTORY":{"onto_name":"constrained iterative deconvolution","onto_id":"FBbi:00000360"},"CELLLINE":{"onto_name":"HeLa S3","onto_id":"MCC:0000220"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Z-focal series of HeLa cells at metaphase. Cells were stained for DNA (blue), phosphorylated mitotic centromere-associated kinesin (MCAK) (red) and HEC1 (green). \nCells were transfected with control (this image) or Bod1 (CIL:13379) siRNA. After 72 h, cells were treated with monastrol for 3 h and released into media containing MG132 for 1 h before fixing."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"chromosome, centromeric region","onto_id":"GO:0000775"},{"free_text":"DNA"},{"free_text":"phosphorylated MCAK"},{"free_text":"HEC1"},{"onto_name":"nuclear chromosome","onto_id":"GO:0000228"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"11195","TECHNICALDETAILS":{"free_text":"Antibodies were rabbit anti-HEC1 (green) antibody (Abcam) used at 1:1,000.  and anti\u2013phospho-MCAK antibodies (red) (Andrews et al., 2004) used at 1 μg\/ml, and DNA (blue).  Fluorescently labeled secondary antibodies were all obtained from Jackson ImmunoResearch Laboratories.  Corresponds to Fig 5B in JCB vol. 179 no. 2 187-197."},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":346,"Pixel_size":{"unit":"microns","value":0.0629},"axis":"X"}},{"Space":{"Image_size":348,"Pixel_size":{"unit":"microns","value":0.0629},"axis":"Y"}},{"Space":{"Image_size":64,"Pixel_size":{"unit":"microns","value":0.2},"axis":"Z"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":{"onto_name":"chemically fixed tissue","onto_id":"FBbi:00000002"},"VISUALIZATIONMETHODS":{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"}}},"Citation":{"Title":"Iain M. Porter, Sarah E. McClelland, Guennadi A. Khoudoli, Christopher J. Hunter, Jens S. Andersen, Andrew D. McAinsh, J. Julian Blow, Jason R. Swedlow (2011) CIL:13378, Homo sapiens. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13378","DOI":"doi:10.7295\/W9CIL13378"}}}