{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1328677200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"39062.jpg","Size":112227,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"39062.tif","Size":1400000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"39062_orig.jpg","Size":200207,"Mime_type":"image\/jpeg"},{"File_type":"Zip","File_path":"39062.zip","Size":200345,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"Wellcome Images","Href":"http:\/\/images.wellcome.ac.uk\/"}],"Contributors":["Spike Walker"]},"BIOLOGICALPROCESS":{"onto_name":"seminiferous tubule development","onto_id":"GO:0072520"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"absorption of illumination","onto_id":"FBbi:00000308"},"IMAGEDESCRIPTION":{"free_text":"This light micrograph shows the outside edge of two seminiferous tubules of a mouse testis. This section is a 1um thick transverse section, stained with toluidine blue to highlight the cells, with the nucleus staining a darker blue. The dark blue line separating the two seminiferous tubules consists mostly of myoid (muscle) tissue. The majority of cells seen in this image (arranged in layers) are germ cells, which, by repeated cell division, eventually produce spermatozoa. Chromosomes are visible in most of the nuclei of the cells. The cell with a deeply-stained nucleus and even darker nucleolus is a Sertoli cell ('nurse cell') that has fine cytoplasmic extensions branching between the other cells to nurture the developing germ cells."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"nucleus","onto_id":"GO:0005634"},{"onto_name":"nuclear chromosome","onto_id":"GO:0000228"},{"onto_name":"nucleolus","onto_id":"GO:0005730"}],"RELATIONTOINTACTCELL":{"onto_name":"sectioned tissue","onto_id":"FBbi:00000026"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"TECHNICALDETAILS":{"free_text":"B0007567 Male germ cells.  Wellcome Images available under the following creative commons usage http:\/\/creativecommons.org\/licenses\/by-nc-nd\/2.0\/uk\/"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_nd"},"IMAGINGMODE":{"onto_name":"modes of light microscopy","onto_id":"FBbi:00000345"},"DIMENSION":[{"Space":{"Image_size":800,"axis":"X"}},{"Space":{"Image_size":500,"axis":"Y"}}],"MOUSE_GROSS_ANATOMY":[{"free_text":"seminiferous tubule"},{"free_text":"myoid tissue"}],"SPECIESTAXASPECIFIC":[{"onto_id":"EMAP:seminiferous tubule"},{"onto_id":"EMAP:myoid tissue"}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":{"onto_name":"chemically fixed tissue","onto_id":"FBbi:00000002"},"VISUALIZATIONMETHODS":{"onto_name":"toluidine blue","onto_id":"FBbi:00000049"},"CELLTYPE":[{"onto_name":"male germ cell","onto_id":"CL:0000015"},{"onto_name":"Sertoli cell","onto_id":"CL:0000216"}]}},"Citation":{"Title":"Spike Walker (2012) CIL:39062, Mus musculus, male germ cell, Sertoli cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil39062","DOI":"doi:10.7295\/W9CIL39062"}}}