{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1297314000},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"13088.jpg","Size":35341,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"13088.zip","Size":12419200,"Mime_type":"application\/zip"},{"File_type":"Mp4","File_path":"13088_web.mp4","Size":462524,"Mime_type":"video\/mp4"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["J Cell Biol 2003 162:377-382"],"Contributors":["Paul Maddox","Aaron Straight","Peg Coughlin","Timothy J. Mitchison","Edward D. Salmon"],"PUBMED":["12900391"]},"BIOLOGICALPROCESS":[{"onto_name":"mitotic anaphase","onto_id":"GO:0000090"},{"free_text":"poleward microtubule flux"}],"PROCESSINGHISTORY":{"free_text":"color combine"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Dual-channel spinning-disk confocal time-lapse movie showing anaphase relative to poleward microtubule flux. Fluorescent speckle microscopy (FSM) of microtubules was performed as described in Maddox et al. (2002, 2003), and with a 100X\/1.4 NA Plan Apochromat objective (Nikon) with 2X2 binning in the cooled CCD camera (model ER; Hamamatsu Corporation). MetaMorph® software (Universal Imaging Corp.) was used to control shutters, wavelength selection, image acquisition, and storage. DNA was visualized by adding Oli-green to the extract (final concentration of 1 µg\/ml). Images were acquired at 10-s intervals. Sequential images of different fluorophores were acquired at 3-15s intervals, depending on the experiment. Paired images from different color channels were within 0.5 s of each other.  Movie is video 1 from J Cell Biol 2003 162:377-382."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"spindle microtubule","onto_id":"GO:0005876"},{"onto_name":"nuclear chromosome","onto_id":"GO:0000228"}],"RELATIONTOINTACTCELL":{"free_text":"cell extract"},"SOURCEOFCONTRAST":[{"free_text":"distribution of nucleic acid"},{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"}],"GROUP_ID":"3925","DATAQUALIFICATION":{"free_text":"RAW;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":[{"onto_name":"spinning disk confocal microscopy","onto_id":"FBbi:00000253"},{"free_text":"fluorescent speckle microscopy"}],"DIMENSION":[{"Space":{"Image_size":454,"Pixel_size":{"unit":"microns","value":0.019},"axis":"X"}},{"Space":{"Image_size":397,"Pixel_size":{"unit":"microns","value":0.019},"axis":"Y"}},{"Wavelength":{"unit":"microns","value":"Oligreen, XRhodamine"}},{"Time":{"unit":"seconds","value":10}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Xenopus [NCBITaxon:262014]","onto_id":"NCBITaxon:262014"},"PREPARATION":{"onto_name":"unfixed tissue","onto_id":"FBbi:00000014"},"VISUALIZATIONMETHODS":[{"onto_name":"X-Rhodamine","onto_id":"FBbi:00000584"},{"onto_name":"probes for nucleic acid","onto_id":"FBbi:00000406"}]}},"Citation":{"Title":"Paul Maddox, Aaron Straight, Peg Coughlin, Timothy J. Mitchison, Edward D. Salmon (2011) CIL:13088, Xenopus [NCBITaxon:262014]. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13088","DOI":"doi:10.7295\/W9CIL13088"}}}