Ris, Hans (2021). CIL:25316, Drosophila melanogaster, fat body cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0X63KX5

Fat body cells from larvae of Drosophila melanogaster were dissected, fixed with formalin-acetic acid, and squashed on a glass slide. After freezing in liquid nitrogen, the cover slips removed, and the slide thawed and stained with 1% uranyl acetate, rinsed in 50% ethanol, dehydrated, transferred to amyl acetate, and covered with 2% parlodion in amyl acetate. Selected areas of the parlodion film, are transferred to formvar coated EM grids, and the parlodion removed by immersion in amyl acetate (see Ris H, 1981 Stereoscopic Electron Microscopy of Chromosomes, Meth Cell Biol 22:77-96). Grids were observed using the Madison High Voltage Electron Microscope at 1 MeV.

Image shows a series of bands and interbands in a polytene chromosome. This image was taken with a tilt angle of 45 degrees. The accompanying grouped image, taken at 55 degrees forms a stereo pair, providing an oblique 3D view of the chromosome.

• 2021

• Ris, Hans

Preparation: formaldehyde fixed tissue; acetic acid treatment
Relation to intact cell: squashed preparation
Item type: film
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data

• Cell Image Library Group ID: 6821

• Drosophila melanogaster

• Fat body cell
• Polytene chromosome

• data
• image

• No linguistic content; Not applicable

Samplenumber: 25316

Doi: https://doi.org/10.6075/J0X63KX5

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL25316

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2022-08-12