{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1588551752},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"CORE":{"TECHNICALDETAILS":{"free_text":"Treated cells were cultured in YPD medium and high pressure frozen in a Wohlwend Compact 3 machine,\r\n followed by a short freeze substitution protocol in 2% Uranyl acetate and embedded in HM20 resin. Sections of 70 nm thickness were then contrast stained with 2% Uranyl acetate and Reynold's lead \r\ncitrate. Pictures were aquired in a Tecnai T12 TEM with a Ceta CMOS 16M camera."},"TERMSANDCONDITIONS":{"free_text":"attribution_cc_by"},"ATTRIBUTION":{"Contributors":["Johanna Höög","Dimitra Panagaki","Jacob Croft"]},"IMAGINGMODE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"}],"BIOLOGICALPROCESS":[{"free_text":"Nuclear envelope budding"}],"IMAGEDESCRIPTION":{"free_text":"Wild type Saccharomyces cerevisiae cells (S228C background) were treated with 0.5 mM of sodium arsenite for 60 minutes. Then, samples were high pressure frozen and visualized through transmission electron microscopy (TEM). The images show the nucleus of the cells and the black arrow indicates nuclear envelope budding events."},"NCBIORGANISMALCLASSIFICATION":[{"free_text":"Saccharomyces cerevisiae  (baker's yeast, budding yeast)"}],"CELLULARCOMPONENT":[{"onto_name":"nucleus","onto_id":"GO:0005634"}],"VISUALIZATIONMETHODS":[{"free_text":"Stain with Uranyl acetate and Lead citrate"}],"GROUP_ID":"20021","CELLTYPE":[{"free_text":"Mixed population of  S. cerevisiae cells"}]},"Image_files":[{"File_type":"Jpeg","File_path":"50843.jpg","Size":1239239,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"50843.zip","Size":3810455,"Mime_type":"application\/zip"}]},"Citation":{"Title":"Johanna Höög;Dimitra Panagaki;Jacob Croft (2020) CIL:50843, Saccharomyces cerevisiae  (baker's yeast, budding yeast), Mixed population of  S. cerevisiae cells. CIL. Dataset. CIL. Dataset","ARK":"ark:\/b7295\/w9cil50843","DOI":"doi:10.7295\/W9CIL50843"}}}