{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1321851600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"38856.tif","Size":100000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"38856.jpg","Size":6015,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"38856.zip","Size":71946,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["S Vitha et al. 2001 FtsZ ring formation at the chloroplast division site in plants. J Cell Biol 153:111-119"],"Contributors":["Stanislav Vitha","Rosemary S McAndrew","Katherine W Osteryoung"],"PUBMED":["11285278"]},"BIOLOGICALPROCESS":[{"onto_name":"chloroplast organization","onto_id":"GO:0009658"},{"onto_name":"plastid organization","onto_id":"GO:0009657"}],"PARAMETERIMAGED":[{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},{"onto_name":"optical path length gradient","onto_id":"FBbi:00000312"}],"IMAGEDESCRIPTION":{"free_text":"Leaves from wildtype Arabidopsis thaliana were fixed, embedded, sectioned, reacted with antibodies to the FtsZ2-1 protein, a prokaryotic cell division protein that is a structural homolog of tubulin.  The protein is localized at the site of division by constricting at the chloroplast mid-point. The upper images show the fluorescence signal, the lower panels, the corresponding DIC image."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"chloroplast","onto_id":"GO:0009507"},{"onto_name":"plastid part","onto_id":"GO:0044435"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":[{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"},{"onto_name":"boundaries between regions with different refractive index","onto_id":"FBbi:00000599"}],"GROUP_ID":"11385","TECHNICALDETAILS":{"free_text":"Leaves were fixed in FAA (formalin acetic acid), embedded in low melting point polyester wax, and 7 micrometer sections cut and attached to poly-L-lysine slides. Sections were de-waxed, treated with an antigen retrieval procedure, and processed for immunofluorescence using incubation with an affinity purified polyclonal antibody, followed by FITC-conjugated secondary antibody. Slides were viewed with an Olympus BH2 microscope equipped with DIC optics.  Images were recorded on film, or a video camera (Optronics DEI 750 using a 100x 1.25 NA objective lens. See S. Vitha et al. 2001 J Cell Biol 153:111-119."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":[{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},{"onto_name":"differential interference contrast microscopy","onto_id":"FBbi:00000245"}],"DIMENSION":[{"Space":{"Image_size":157,"axis":"X"}},{"Space":{"Image_size":152,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Arabidopsis thaliana","onto_id":"NCBITaxon:3702"},"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"tissue in wax embedment","onto_id":"FBbi:00000019"}],"VISUALIZATIONMETHODS":{"onto_name":"Fluorescein (FITC)","onto_id":"FBbi:00000451"},"CELLTYPE":{"free_text":"leaf"}}},"Citation":{"Title":"Stanislav Vitha, Rosemary S McAndrew, Katherine W Osteryoung (2011) CIL:38856, Arabidopsis thaliana, leaf. CIL. Dataset","ARK":"ark:\/b7295\/w9cil38856","DOI":"doi:10.7295\/W9CIL38856"}}}