{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1331182800},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"40377.jpg","Size":88100,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"40377.zip","Size":71225472,"Mime_type":"application\/zip"},{"File_type":"Mp4","File_path":"40377_web.mp4","Size":709096,"Mime_type":"video\/mp4"}],"CORE":{"ATTRIBUTION":{"Contributors":["Patricia Wadsworth","U Serder Tulu"]},"BIOLOGICALPROCESS":[{"onto_name":"mitosis","onto_id":"GO:0007067"},{"onto_name":"microtubule cytoskeleton organization","onto_id":"GO:0000226"},{"free_text":"cell cycle"}],"CELLLINE":{"onto_name":"LLC-PK1","onto_id":"MCC:0000286"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Time lapse movie of LLCPK cells stably expressing GFP-EB1, a microtubule tip binding protein. GFP-EB1 fluorescence is seen in microtubules and centrosomes which appear to act as microtubule nucleation sites.\nThe stack of images used to prepare the movie is included in this group of images."},"ITEMTYPE":{"onto_name":"charge coupled device (CCD)","onto_id":"FBbi:00000294"},"CELLULARCOMPONENT":[{"free_text":"microtubule"},{"onto_name":"microtubule cytoskeleton","onto_id":"GO:0015630"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"10940","TECHNICALDETAILS":{"free_text":"See: KJ Salaycik et al. 2005. Quantification of microtubule growth and dynamics in wound-edge cells. J Cell Sci 118:4113-4122.\nhttp:\/\/jcs.biologists.org\/content\/118\/18\/4113.long"},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"spinning disk confocal microscopy","onto_id":"FBbi:00000253"},"DIMENSION":[{"Space":{"Image_size":650,"axis":"X"}},{"Space":{"Image_size":515,"axis":"Y"}},{"Time":{"unit":"seconds","value":2,"frame":"93"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Sus scrofa domestica","onto_id":"NCBITaxon:9825"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"EGFP","onto_id":"FBbi:00000082"},"CELLTYPE":{"onto_name":"kidney epithelial cell","onto_id":"CL:0002518"}}},"Citation":{"Title":"Patricia Wadsworth, U Serder Tulu (2012) CIL:40377, Sus scrofa domestica, kidney epithelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil40377","DOI":"doi:10.7295\/W9CIL40377"}}}