Jin, Seok Min; Lazarou, Michael; Wang, Chunxin; Kane, Lesley A.; Narendra, Derek P.; Youle, Richard J. (2021). CIL:13734, Homo sapiens, endothelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J03X85CQ

Mitochondria in HeLa cells are identified by mito-YFP (green). When fixed cells are permeabilized with 0.25% Triton X-100 (TX-100), both antibodies to cyctochrome c (red), a mitochondrial matrix marker, and the antibody to Tom20 (white), a mitochondrial outer membrane marker, are able to bind their epitopes. Mito-YFP transfected HeLa cells were treated with DMSO for 3 hrs, fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton X-100/PBS. Primary antibodies used were: anti-Cyt c mAb (BD) and anti-Tom20 mAb (BD); secondary antibodies used were: Alexa Fluor 594 and 647. Imaging was performed on an LSM510 Meta (Carl Zeiss, Inc) with a 63x 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the LSM image browser (Zeiss). This image corresponds to Fig 4b, 3rd row from top and is part of a differential permeabilization assay with and without the mitochondrial depolarizing agent CCCP (carbonyl cyanide m-chlorophenyl hydrazone) that is further described in Fig 4b of J Cell Biol, 191: 933-942, 2010. Images in Fig 4 include CIL#s 13733, 13734, 13729, 13730, 13731, 13732, 13717, 13718, 13719, 13720, 13721, 13722, 13723, 13724.

• 2021

• Jin, Seok Min
• Kane, Lesley A.
• Lazarou, Michael
• Narendra, Derek P.
• Wang, Chunxin
• Youle, Richard J.

Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EYFP; primary antibody plus labeled secondary antibody
Processing history: brightness and contrast adjusted
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 7051

• Homo sapiens

• Endothelial cell
• Mitochondrial intermembrane space
• Mitochondrial outer membrane translocase complex
• Mitochondrion

• Cervical carcinoma
• Electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity
• HeLa
• Heme binding
• P-P-bond-hydrolysis-driven protein transmembrane transporter activity
• Protein targeting to mitochondrion
• Respiratory electron transport chain

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13734

Doi: https://doi.org/10.6075/J03X85CQ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13734

Reference

• J Cell Biol, 191: 933-942, 2010. https://www.ncbi.nlm.nih.gov/pubmed/?term=21115803

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

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2022-08-12