{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1295154000},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"12299.tif","Size":10600000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"12299.jpg","Size":3197508,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"12299.zip","Size":21005194,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/www5.pbrc.hawaii.edu\/allen\/"}],"Contributors":["M. Sage","Richard Allen"]},"BIOLOGICALPROCESS":[{"onto_name":"cortical cytoskeleton organization","onto_id":"GO:0030865"},{"onto_name":"cytoplasm organization","onto_id":"GO:0007028"},{"onto_name":"plasma membrane organization","onto_id":"GO:0007009"},{"onto_name":"detection of symbiont","onto_id":"GO:0009602"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"free_text":"Print from negative scanned to Photoshop."}],"CELLLINE":{"free_text":"Carolina Biological Supply Company, NC, U.S.A."},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"A dividing Didinium nasutum. This large ciliate is capable of engulfing Paramecium. It has a thick ectoplasm containing numerous mucocysts embedded in its extensive epiplasm. Its external surface is coated with a layer of bacteria in this dividing cell. The cytoplasm is relatively compact under the conditions of fixation used. Electron opaque vacuoles are scattered in the cytoplasm. (For a more detailed report see Wessenberg and Antipa, Protistologica 4:427-447, 1968). TEM taken on 5\/9\/69 by M. Sage with Philips 300 operating at 60kV. Neg. 3,120X. Bar = 2µm."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"cell cortex","onto_id":"GO:0005938"},{"onto_name":"cytoplasm","onto_id":"GO:0005737"},{"free_text":"extrusomes"},{"free_text":"ectosymbiont"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"9772","TECHNICALDETAILS":{"free_text":"The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. A high resolution view suitable for qunatitative analysis is available in the library (CIL:38862). Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"DATAQUALIFICATION":{"free_text":"PROCESSED;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":2805,"axis":"X"}},{"Space":{"Image_size":3744,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Didinium nasutum","onto_id":"NCBITaxon:5997"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"M. Sage, Richard Allen (2011) CIL:12299, Didinium nasutum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil12299","DOI":"doi:10.7295\/W9CIL12299"}}}