{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1593056817},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"CORE":{"TECHNICALDETAILS":{"free_text":"Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample. <br><br>\r\n5.1_Cell_5_Raw_Image_Stack is a multipage TIFF file consisting of 233 individual EM sections acquired at 20 nm milling step, resulting in 2.75×2.75×20 nm³ voxel size. <br><br>\r\nThis file is a part of Dataset 5, comprised of files related to the Cell #5 of current study. The numbering order of the files reflects the order of operations performed during the analysis."},"TERMSANDCONDITIONS":{"free_text":"attribution_cc_by"},"ATTRIBUTION":{"OTHER":["Massachusetts Eye and Ear; Harvard Medical School","5.1_Cell_5_Raw_Image_Stack"],"URLs":[{"Label":"Scientific Data article","Href":"https:\/\/www.nature.com\/articles\/s41597-020-0509-4"},{"Label":"Nature Communications article","Href":"https:\/\/www.nature.com\/articles\/s41467-019-11712-w"}],"Contributors":["Maryna V. Ivanchenko","Marcelo Cicconet","Hoor Al Jandal","Xudong Wu","David P. Corey (<a href='http:\/\/corey.med.harvard.edu\/' target='_blank'>http:\/\/corey.med.harvard.edu\/<\/a>)","Artur A. Indzhykulian (<a href='https:\/\/ailab.hms.harvard.edu\/' target='_blank'>https:\/\/ailab.hms.harvard.edu\/<\/a>)"]},"IMAGINGMODE":[{"free_text":"Serial Electron Microscopy"},{"free_text":"Focused Ion Beam Scanning Electron Microscopy (FIB-SEM)"}],"BIOLOGICALPROCESS":[{"free_text":"Native auditory Hair Cells at postnatal day 4"}],"IMAGEDESCRIPTION":{"free_text":"FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. Postnatal day 4 mouse Inner Ear Organ of Corti labeled with anti-PKHD1L1 antibody (10 nm gold beads). The dataset represents the apical end of the Outer Hair Cell with the stereocilium bundle. Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample."},"NCBIORGANISMALCLASSIFICATION":[{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"}],"CELLULARCOMPONENT":[{"free_text":"Hair Cell stereocilia"},{"free_text":"Cuticular plate"},{"free_text":"Mitochondria "},{"free_text":"Polycystic Kidney and Hepatic Disease 1-Like 1 (PKHD1L1) labeling (gold beads)"}],"VISUALIZATIONMETHODS":[{"free_text":"Heavy metal stain (Osmium Tetroxide, Potassium Ferrocyanide, Tannic Acid)"},{"free_text":"Immunogold labeling with 10 nm gold beads"}],"CELLTYPE":[{"free_text":"Inner Ear Outer Hair Cell"}],"GROUP_ID":"20005"},"Image_files":[{"File_type":"Jpeg","File_path":"50713.jpg","Size":224146,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"50713.zip","Size":1465952875,"Mime_type":"application\/zip"}]},"Citation":{"Title":"Maryna V. Ivanchenko, Marcelo Cicconet, Hoor Al Jandal, Xudong Wu, David P. Corey, Artur A. Indzhykulian (2019). FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles: 5.1_Cell_5_Raw_Image_Stack. CIL","ARK":"ark:\/b7295\/w9cil50713","DOI":"doi:10.7295\/W9CIL50713"}}}