{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1307246400},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"13600.jpg","Size":217501,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"13600.tif","Size":4400000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"13600.zip","Size":4352660,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["JCB 189: 1157-1169, 2010"],"Contributors":["Yuting Sun","Yejing Ge","Jenny Dmevich","Yong Zhao","Mark Band","Jie Chen"],"PUBMED":["20566686"]},"BIOLOGICALPROCESS":{"onto_name":"myoblast fusion involved in skeletal muscle regeneration","onto_id":"GO:0014905"},"PROCESSINGHISTORY":{"free_text":"Brightness and Contrast Adjusted"},"PARAMETERIMAGED":{"onto_name":"absorption of illumination","onto_id":"FBbi:00000308"},"IMAGEDESCRIPTION":{"free_text":"Mouse hind limb tibialis anterior (TA) muscles were injected with BaCl2 followed by daily systemic administration of rapamycin (Rap) starting 7 days after injury. The animals were sacrificed at different days, and the injured muscles were dissected and cryosectioned followed by hematoxylin (blue) and eosin (pink to red) staining.\n\nThis image shows the regeneration of muscle tissue fourteen days after the injury with daily systemic administration of Rap and trichostatin A (TSA) from day 7 to day 13. The growth inhibition of myofibers by Rap was rescued by TSA. Myofibers were stained by eosin in red and nuclei were stained by hematoxylin in blue. This image corresponds to Fig 10B from JCB 189: 1157-1169, 2010. See also CIL: 13595, 13596, 13597, 13598, and 13599."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"nucleus","onto_id":"GO:0005634"},"RELATIONTOINTACTCELL":{"onto_name":"sectioned tissue","onto_id":"FBbi:00000026"},"SOURCEOFCONTRAST":[{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},{"onto_name":"compartmentalization of stain or label","onto_id":"FBbi:00000604"}],"GROUP_ID":"8415","MOLECULARFUNCTION":[{"free_text":"mTOR inhibition"},{"onto_name":"histone deacetylase inhibitor activity","onto_id":"GO:0046811"}],"TECHNICALDETAILS":{"free_text":"TA muscles were isolated by dissection, frozen in liquid nitrogen\u2013cooled 2-methylbutane, and embedded in TBS tissue freezing medium. Sections of 10-μm thickness were made with a cryostat at -20°C, placed on uncoated slides, and stained with hematoxylin and eosin. The stained slides were examined with a microscope (DMI 4000B), and the images were captured with a Fluotar 20× 0.4 NA dry objective (Leica) using a camera (RETIGA Exi). The images were processed as 24-bit colored images using Photoshop."},"DATAQUALIFICATION":{"free_text":"PROCESSED;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"bright-field microscopy","onto_id":"FBbi:00000243"},"DIMENSION":[{"Space":{"Image_size":1392,"Pixel_size":{"unit":"microns","value":0.32},"axis":"X"}},{"Space":{"Image_size":1040,"Pixel_size":{"unit":"microns","value":0.32},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":[{"onto_name":"cryostat-sectioned tissue","onto_id":"FBbi:00000027"},{"free_text":"Fix in liquid nitrogen\u2013cooled 2-methylbutane"}],"VISUALIZATIONMETHODS":[{"onto_name":"hematoxylin","onto_id":"FBbi:00000041"},{"onto_name":"eosin","onto_id":"FBbi:00000037"}],"CELLTYPE":{"onto_name":"skeletal muscle cell","onto_id":"CL:0000188"}}},"Citation":{"Title":"Yuting Sun, Yejing Ge, Jenny Dmevich, Yong Zhao, Mark Band, Jie Chen (2011) CIL:13600, Mus musculus, skeletal muscle cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13600","DOI":"doi:10.7295\/W9CIL13600"}}}