{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1287892800},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Mp4","File_path":"8059_web.mp4","Size":2711894,"Mime_type":"video\/mp4"},{"File_type":"Jpeg","File_path":"8059.jpg","Size":26667,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"8059.zip","Size":4714620,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Mardones, Gonzalo A."]},"BIOLOGICALPROCESS":{"onto_name":"secretion","onto_id":"GO:0046903"},"PROCESSINGHISTORY":{"onto_name":"non-linear methods","onto_id":"FBbi:00000355"},"CELLLINE":{"onto_name":"NRK-52E","onto_id":"MCC:0000366"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Brefeldin A (BFA) is a fungal metabolite that reversibly blocks protein transport from the endoplasmic reticulum (ER) to the Golgi apparatus. This effect is accompanied by a distinctive morphological change, the reversible collapse of the Golgi apparatus. The disassembly of the Golgi apparatus is accompanied by its dramatic tubulation, forming a network extending throughout the cytoplasm. While the content of the Golgi apparatus is redistributed to the ER, the content of the trans-Golgi network (TGN) collapses in the vicinity of the MTOC. \n\nThis video shows the effect of BFA in an NRK cell stably expressing GFP-GMx33a, a protein associated to the TGN. \n\nNRK cells stably expressing GFP-GMx33a and plated on 35-mm glass-bottom culture dishes (MatTek), were treated with 5 microgram\/ml BFA and maintained in phenol red-free buffered medium at 37C by using an ASI 400 Air Stream stage Incubator (Nevtek, Burnsville, VA). After 10 minutes in the presence of BFA time-lapse fluorescence images were acquired with an Ultraview Confocal Scanner (Perkin Elmer-Cetus, Norwalk, CT) in a Nikon Eclipse TE2000-S inverted microscope (Nikon, Melville, NY) equipped with a PlanApo 100x oil immersion objective (NA 1.40; Nikon), and a 12-bit charge-coupled device (CCD) camera, ORCA (Hamamatsu, Bridgewater, NJ). Image capture and data acquisition were performed using Ultraview LCI software (Perkin Elmer-Cetus). Images were acquired in binning 2 x 2 modes at approximately 0.25-s intervals, during approximately 175 s. Sequence images were exported as single TIFF files, and processed with ImageJ 1.36b (Rasband, 1997, 2007; http:\/\/rsb.info.nih.gov\/ij\/).\t\n\nLippincott-Schwartz J, Donaldson JG, Schweizer A, Berger EG, Hauri HP, Yuan LC, Klausner RD. Microtubule-dependent retrograde transport of proteins into the ER in the presence of brefeldin A suggests an ER recycling pathway. Cell [serial online]. 1990;60:821-36. \n\nLippincott-Schwartz J, Yuan L, Tipper C, Amherdt M, Orci L, Klausner RD. Brefeldin A's effects on endosomes, lysosomes, and the TGN suggest a general mechanism for regulating organelle structure and membrane traffic. Cell [serial online]. 1991;67:601-16. \n\nSnyder CM, Mardones GA, Ladinsky MS, Howell KE. GMx33 associates with the trans-Golgi matrix in a dynamic manner and sorts within tubules exiting the Golgi. Mol Biol Cell [serial online]. 2006;17:511-24."},"ITEMTYPE":{"onto_name":"confocal microscopy","onto_id":"FBbi:00000251"},"CELLULARCOMPONENT":{"onto_name":"Golgi apparatus part","onto_id":"GO:0044431"},"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"1564","TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":328,"Pixel_size":{"unit":"nanometers","value":150},"axis":"X"}},{"Space":{"Image_size":392,"Pixel_size":{"unit":"nanometers","value":150},"axis":"Y"}},{"Time":{"unit":"seconds","value":175}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Rattus norvegicus","onto_id":"NCBITaxon:10116"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":[{"onto_name":"fluorescent protein tag","onto_id":"FBbi:00000405"},{"onto_name":"Green fluorescent proteins from Aequorea","onto_id":"FBbi:00000437"}],"CELLTYPE":{"onto_name":"permanent cell line cell","onto_id":"CL:0000002"}}},"Citation":{"Title":"Mardones, Gonzalo A. (2010) CIL:8059, Rattus norvegicus, permanent cell line cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil8059","DOI":"doi:10.7295\/W9CIL8059"}}}