{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1306987200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"35127.tif","Size":2900000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"35127.jpg","Size":129560,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"35127.zip","Size":2870678,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Michael Cammer","Dianne Cox"]},"BIOLOGICALPROCESS":{"onto_name":"ruffle organization","onto_id":"GO:0031529"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"onto_name":"Bone marrow","onto_id":"FMA:9608"},"PARAMETERIMAGED":{"onto_name":"elastic scattering of photons","onto_id":"FBbi:00000587"},"IMAGEDESCRIPTION":{"free_text":"Primary bone marrow macrophages derived from mouse plated on a coverslip and imaged live.  Phase contrast microscopy allows us to see the dense f-actin rich leading edge, ruffles, mitochondria, the nucleus, and other organelles throughout the volume of the cells.  At the center of the image is a polarized lamellipod of a crawling cell.  The glass micropipette at the bottom of the image is delivering CSF (colony stimulating factor) locally and the cell may be responding."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"ruffle membrane","onto_id":"GO:0032587"},"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"differences in amount of elastic light scattering","onto_id":"FBbi:00000600"},"GROUP_ID":"8398","MOLECULARFUNCTION":{"onto_name":"chemokine receptor activity","onto_id":"GO:0004950"},"TECHNICALDETAILS":{"free_text":"Live bone marrow macrophages plated in MatTek dish and imaged with a 60X N.A. 1.4 phase 3 Olympus objective on an IX71 microscope with a Cooke Sensicam QE camera and IPLab software running on a Dell Windows XP computer."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"phase contrast microscopy","onto_id":"FBbi:00000247"},"DIMENSION":[{"Space":{"Image_size":1376,"Pixel_size":{"unit":"microns","value":0.11},"axis":"X"}},{"Space":{"Image_size":1040,"Pixel_size":{"unit":"microns","value":0.11},"axis":"Y"}}],"MOUSE_GROSS_ANATOMY":[{"onto_name":"TS24,femur","onto_id":"EMAP:12912"}],"SPECIESTAXASPECIFIC":{"onto_id":"EMAP:EMAP:12912"},"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"visualization of contiguous regions","onto_id":"FBbi:00000396"},"CELLTYPE":{"onto_name":"macrophage","onto_id":"CL:0000235"},"PATHOLOGICALPROCESS":{"onto_id":"IDO:IDO:0000012"}}},"Citation":{"Title":"Michael Cammer, Dianne Cox (2011) CIL:35127, Mus musculus, macrophage. CIL. Dataset","ARK":"ark:\/b7295\/w9cil35127","DOI":"doi:10.7295\/W9CIL35127"}}}