{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1332648000},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"40470.tif","Size":12600000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"40470.zip","Size":12583716,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"40470.jpg","Size":915684,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"Human Protein Atlas","Href":"http:\/\/www.proteinatlas.org\/ENSG00000167916\/subcellular"}],"PUBLISHED":["Nat Biotechnol. 2010 28(12):1248-50."],"Contributors":["M Uhlen","P Oksvold","L Fagerberg","E Lundberg","K Jonasson","M Forsberg","M Zwahlen","C Kampf","K Wester","S Hober","H Wernerus","L Björling","F Ponten"],"PUBMED":["21139605"]},"BIOLOGICALPROCESS":[{"onto_name":"organelle organization","onto_id":"GO:0006996"},{"onto_name":"cytoskeleton organization","onto_id":"GO:0007010"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"onto_name":"U-2 OS","onto_id":"MCC:0000468"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Single slice confocal images representing one optical slice of a cell stained for the Human Protein Atlas (HPA) antibody HPA022978 (shown in green). HPA022978 is a polyclonal anti-KRT24 antibody that labels intermediate filaments in these cells. Thee cells are also stained with reference markers in order to facilitate the annotation of the subcellular distribution of the protein targeted by the HPA antibody. The following probes\/organelles are used as references; (i) DAPI for the nucleus (blue) and (ii) anti-tubulin antibody as internal control and marker of microtubules (red)."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"intermediate filament","onto_id":"GO:0005882"},{"onto_name":"nucleus","onto_id":"GO:0005634"},{"onto_name":"microtubule cytoskeleton","onto_id":"GO:0015630"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"},"GROUP_ID":"11226","TECHNICALDETAILS":{"free_text":"The use of data and images from this site in publications and presentations is permitted provided that the following conditions are met:\n\nThe publication and\/or presentation are solely for informational and non-commercial purposes.\n\nThe source of the data and\/or image is referred to this site (www.proteinatlas.org) and\/or the above publication is cited."},"DATAQUALIFICATION":{"free_text":"RAW"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"confocal microscopy","onto_id":"FBbi:00000251"},"DIMENSION":[{"Space":{"Image_size":2048,"axis":"X"}},{"Space":{"Image_size":2048,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":[{"onto_name":"chemically fixed tissue","onto_id":"FBbi:00000002"},{"onto_name":"permeabilized tissue","onto_id":"FBbi:00000093"}],"VISUALIZATIONMETHODS":{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},"CELLTYPE":{"free_text":"osteosarcoma"}}},"Citation":{"Title":"M Uhlen, P Oksvold, L Fagerberg, E Lundberg, K Jonasson, M Forsberg, M Zwahlen, C Kampf, K Wester, S Hober, H Wernerus, L Björling, F Ponten (2012) CIL:40470, Homo sapiens, osteosarcoma. CIL. Dataset","ARK":"ark:\/b7295\/w9cil40470","DOI":"doi:10.7295\/W9CIL40470"}}}