{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1295413200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"12315.jpg","Size":2226124,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"12315.zip","Size":20411786,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"12315.tif","Size":10500000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/www5.pbrc.hawaii.edu\/allen\/"}],"Contributors":["M. Aihara","Richard Allen (University of Hawaii)"]},"BIOLOGICALPROCESS":[{"onto_name":"cortical cytoskeleton organization","onto_id":"GO:0030865"},{"onto_name":"clathrin coat assembly","onto_id":"GO:0048268"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"free_text":"Print from negative scanned for Photoshop"}],"CELLLINE":{"free_text":"Cambridge Culture Collection, Cambridge, England, catalog # LB1650\/1"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Surface of Nassula aurea. Early endosomes and clathrin coated vesicles. One clathrin coated pit at the bottom of a parasomal sac is shown. TEM taken on 2\/22\/71 by M. Aihara with Hitachi HU11A operating at 50kV. Neg. 10,750X. Bar = 0.5µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:7666) is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm.\n\nAdditional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"cell cortex","onto_id":"GO:0005938"},{"onto_name":"clathrin coat","onto_id":"GO:0030118"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"4832","DATAQUALIFICATION":{"free_text":"PROCESSED;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":3229,"axis":"X"}},{"Space":{"Image_size":3222,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Nassula","onto_id":"NCBITaxon:502083"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"M. Aihara, Richard Allen (University of Hawaii) (2011) CIL:12315, Nassula, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil12315","DOI":"doi:10.7295\/W9CIL12315"}}}