{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1333425600},"Data_type":{"Still_image":false,"Z_stack":false,"Video":false,"Time_series":true},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"40658.jpg","Size":21972,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"40658.tif","Size":3800000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"40658.zip","Size":3769417,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Vladimir Gelfand","Urko del Castillo"]},"BIOLOGICALPROCESS":[{"onto_name":"microtubule cytoskeleton organization","onto_id":"GO:0000226"},{"free_text":"microtubule"}],"CELLLINE":{"free_text":"S2"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Time series spinning disc confocal images of Drosophila S2 cells stably expressing pMT-Eos2-alpha tubulin. A diagonal ~2.5 micrometer strip was photoconverted and images taken every minute for 20 minutes. Microtubules clearly migrate beyond the confines of the photoconverted strip.\nThis image stack is part of a grouped set that includes a corresponding time lapse movie."},"ITEMTYPE":{"onto_name":"electron multiplying CCD (EMCCD)","onto_id":"FBbi:00000301"},"CELLULARCOMPONENT":[{"onto_name":"microtubule","onto_id":"GO:0005874"},{"onto_name":"cytoskeleton","onto_id":"GO:0005856"}],"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"GROUP_ID":"11370","TECHNICALDETAILS":{"free_text":"Time series were recorded using a Nikon U-2000 inverted microscope equipped with a Nikon 100x NA 1.4 objective lens, Perfect Focus System, CSU-10 spinning disc confocal head (Yokogawa) and Evolve EMCCD camera (Roper Scientific) driven by Nikon Elements software. \nSee also: A.L. Jolly et al. 2010 Proc Natl Acad Sci USA 107:12151-12156."},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"spinning disk confocal microscopy","onto_id":"FBbi:00000253"},"DIMENSION":[{"Space":{"Image_size":411,"axis":"X"}},{"Space":{"Image_size":456,"axis":"Y"}},{"Time":{"unit":"seconds","value":60,"frame":"20"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Drosophila melanogaster","onto_id":"NCBITaxon:7227"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"mEOS2FP","onto_id":"FBbi:00000552"}}},"Citation":{"Title":"Vladimir Gelfand, Urko del Castillo (2012) CIL:40658, Drosophila melanogaster. CIL. Dataset","ARK":"ark:\/b7295\/w9cil40658","DOI":"doi:10.7295\/W9CIL40658"}}}