von Dassow, George; Verbrugghe, Koen J.C.; Miller, Ann L.; Sider, Jenny R.; Bement, William M. (2021). CIL:15784, Dendraster excentricus. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0TQ607J

6-cell sand dollar embryo expressing three tandem GFPs fused to the ensconsin microtubule-binding domain (EMTB-3G; Faire et al., 1999). 10 µM nocodazole was added at time 00:00, at which time three of four macromeres have entered anaphase; the east cell enters anaphase shortly thereafter (03:00). In all cells, numerous astral microtubules, pointing in all directions, persist >5 min after nocodazole addition. Stable microtubules may approach the cortex in west and south cells (03:00), but none can be seen to do so in north and east cells; all commence furrowing at a normal time and complete cytokinesis. Note that in all cases, stable microtubules become brighter because EMTB-3G liberated by disassembly becomes available for binding.
Sand dollar embryos were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens. Room temperature was maintained at 14–17°C, which is tolerated well. Movie corresponds to Fig 3C and fourth segment of video 3 in J Cell Biol. 2009 Dec 14;187(6):831-45.

• 2021

• Bement, William M.
• Miller, Ann L.
• Sider, Jenny R.
• Verbrugghe, Koen J.C.
• Von Dassow, George

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data

• Cell Image Library Group ID: 6184

• Dendraster excentricus

• Astral microtubule
• Cell cortex
• Cleavage furrow
• Ensconsin
• Microtubule

• Cytokinesis after mitosis
• Embryo
• Mitotic anaphase
• Nocodazole treatment

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 15784

Doi: https://doi.org/10.6075/J0TQ607J

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL15784

Reference

• J Cell Biol. 2009 Dec 14;187(6):831-45. https://www.ncbi.nlm.nih.gov/pubmed/?term=20008563

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2022-08-12