{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1416200400},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"47050_orig.jpg","Size":45979,"Mime_type":"image\/jpeg"},{"File_type":"OME_tif","File_path":"47050.tif","Size":800000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"47050.jpg","Size":62451,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"47050.zip","Size":46117,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"OTHER":["Eric Bushong","Mark Ellisman","Da-Yong Lee"],"URLs":[{"Label":"CCDB 7750","Href":"http:\/\/ccdb.ucsd.edu\/sand\/main?mpid=7750&count=y&event=displaySum"}],"Contributors":["Keun-Young Kim","David Gutmann"]},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"The purpose of this study was to investigate the progression of changes in retinal ganglion cells and optic nerve glia in neurofibromatosis-1 (NF1) genetically-engineered mice with optic glioma. RGC were counted in whole retina preparations using high-resolution, mosaic confocal microscopy following their delineation by retrograde FluoroGold labeling. Then, we found reduced RGC numbers in Nf1+\/GFAPCKO mice, supporting a model in which the combination of optic nerve Nf1 heterozygosity and glial cell Nf1 loss results in disrupted axonal-glial relationships, subsequently culminating in the degeneration of optic nerve axons and loss of their parent RGC neurons."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"cell body","onto_id":"GO:0044297"},"GROUP_ID":"17001","TECHNICALDETAILS":{"free_text":"Specimen was imaged using a Olympus DSU Confocal Microscope with the Olympus 20x 1.4 NA objective."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"confocal microscopy","onto_id":"FBbi:00000251"},"DIMENSION":[{"Space":{"Image_size":14000,"Pixel_size":{"unit":"nanometers","value":0.39},"axis":"X"}},{"Space":{"Image_size":14000,"Pixel_size":{"unit":"nanometers","value":0.39},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"CELLTYPE":{"onto_name":"retinal ganglion cell","onto_id":"CL:0000740"}}},"Citation":{"Title":"Keun-Young Kim, David Gutmann (2014) CIL:47050, Mus musculus, retinal ganglion cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil47050","DOI":"doi:10.7295\/W9CIL47050"}}}