{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1291784400},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"9686.zip","Size":21490016,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"9686.tif","Size":21400000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"9686.jpg","Size":482661,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"Contributors":["Martin Wuehr","Timothy J. Mitchison"]},"BIOLOGICALPROCESS":{"onto_name":"meiotic metaphase II","onto_id":"GO:0007137"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Frog (Xenopus laevis) egg arrested in metaphase of mitosis. Note the tiny metaphase spindle on the top left of the egg. \n\nEggs were fixed in 90% MeOH; 10% (H20 500mM EGTA) and stained for DNA with YoPro1 (green) and tubulin (red) with antibody B-5-1-2 directly labeled with Alexa 647.  A single z slice was collected with a Zeiss LSM 710 using a 10 X 0.3NA objective.  Lasers were 488 (DNA stain) and 633 (Tubulin stain)."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"spindle microtubule","onto_id":"GO:0005876"},{"onto_name":"condensed chromosome","onto_id":"GO:0000793"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"GROUP_ID":"7118","TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"single-spot confocal microscopy","onto_id":"FBbi:00000252"},"DIMENSION":[{"Space":{"Image_size":3264,"Pixel_size":{"unit":"microns","value":0.4341},"axis":"X"}},{"Space":{"Image_size":3264,"Pixel_size":{"unit":"microns","value":0.4341},"axis":"Y"}},{"Wavelength":{"unit":"microns","value":"488, 633"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Xenopus laevis","onto_id":"NCBITaxon:8355"},"PREPARATION":{"onto_name":"methanol fixed tissue","onto_id":"FBbi:00000007"},"VISUALIZATIONMETHODS":[{"onto_name":"YO-PRO-1","onto_id":"FBbi:00000068"},{"onto_name":"Alexa Fluor 647","onto_id":"FBbi:00000447"},{"onto_name":"labeled primary antibody","onto_id":"FBbi:00000130"}],"CELLTYPE":{"onto_id":"CL:0000254"}}},"Citation":{"Title":"Martin Wuehr, Timothy J. Mitchison (2010) CIL:9686, Xenopus laevis. CIL. Dataset","ARK":"ark:\/b7295\/w9cil9686","DOI":"doi:10.7295\/W9CIL9686"}}}