{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1306123200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"26555.zip","Size":529233,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"26555.tif","Size":600000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"26555.jpg","Size":112599,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/3112\/"}],"PUBLISHED":["J. Cell Biol. 2010. Vol. 190(4):663\u2013674"],"Contributors":["Chan-Soo Lee","Chang-Ki Choi","Eun-Young Shin","Martin Alexander Schwartz","Eung-Gook Kim"],"PUBMED":["PMID:20713598"]},"BIOLOGICALPROCESS":{"onto_name":"regulation of cell migration","onto_id":"GO:0030334"},"CELLLINE":{"onto_name":"NIH\/3T3","onto_id":"MCC:0000362"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"To study the molecular mechanism by which nonmuscle myosin II (MII) regulates protrusion and adhesion dynamics in migrating cells, NIH3T3 cells were treated with 50 µm blebbistatin (BBS) for 15 min. and stained for βPIX (green) and myosin IIA (red). βPIX staining is transformed into numerous small puncta along the membrane after BBS treatment, and that myosin staining is excluded from the leading edge. These findings help elucidate a functional link between MII and Rac1\/Cdc42 GTPases, which may regulate protrusion\/adhesion dynamics in migrating cells.  This image is the original data file from Fig. S4, \u201CPotential involvement of βPIX  in blebbistatin-induced morphological changes in MII-regulated cell protrusion and adhesion.\u201D J. Cell Biol. 2010. Vol. 190(4):663\u2013674."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"lamellipodium","onto_id":"GO:0030027"},{"onto_name":"myosin II complex","onto_id":"GO:0016460"},{"onto_name":"actin filament","onto_id":"GO:0005884"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"},"GROUP_ID":"8283","TECHNICALDETAILS":{"free_text":"Cells were cultured in DME (Invitrogen) supplemented with 10% fetal bovine serum and 100 U\/ml penicillin\/streptomycin (Invitrogen) at 37°C in a humidified 5% CO2 incubator. Cells were treated with 50 µM blebbistatin, 15min, fixed using 3.7% paraformaldehyde in PBS for 15 min, permeabilized using 0.2% Triton X-100 in PBS for 2 min, blocked with 2% BSA in PBS, and stained with the indicated primary antibodies for at 4°C overnight, followed by incubation with a secondary Alexa Fluor 488\u2013, 546\u2013 or 594\u2013conjugated antibody. F-actin was visualized with TRITC or Alexa Fluor 350\u2013conjugated phalloidin. Images were captured by Leica TCS SP2 confocal microscope with HCX PL APO 63X objective."},"DATAQUALIFICATION":{"free_text":"RAW"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"confocal microscopy","onto_id":"FBbi:00000251"},"DIMENSION":[{"Space":{"Image_size":512,"axis":"X"}},{"Space":{"Image_size":512,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"detergent permeabilized","onto_id":"FBbi:00000262"}],"VISUALIZATIONMETHODS":[{"onto_name":"Alexa Fluor 488","onto_id":"FBbi:00000440"},{"onto_name":"Alexa Fluor 546","onto_id":"FBbi:00000441"}],"CELLTYPE":{"onto_name":"fibroblast","onto_id":"CL:0000057"}}},"Citation":{"Title":"Chan-Soo Lee, Chang-Ki Choi, Eun-Young Shin, Martin Alexander Schwartz, Eung-Gook Kim (2011) CIL:26555, Mus musculus, fibroblast. CIL. Dataset","ARK":"ark:\/b7295\/w9cil26555","DOI":"doi:10.7295\/W9CIL26555"}}}