{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1291093200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"1584.zip","Size":1070186,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"1584.tif","Size":1100000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"1584.jpg","Size":155450,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"Contributors":["Caroline McKeown"]},"BIOLOGICALPROCESS":[{"onto_name":"striated muscle contraction","onto_id":"GO:0006941"},{"onto_name":"cytoskeleton organization","onto_id":"GO:0007010"}],"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Cultured chicken embryonic cardiomyocyte fixed and stained for alpha-actinin using a monoclonal antibody and Alexa-546 as a secondary fluorescent antibody. Imaged by widefield epifluorescence on a Zeiss Axioplan microscope with a 63x objective. Image taken with a Hamamatsu Orca-ER CCD camera. Scale bar is 6um."},"ITEMTYPE":{"onto_name":"charge coupled device (CCD)","onto_id":"FBbi:00000294"},"CELLULARCOMPONENT":[{"onto_name":"striated muscle dense body","onto_id":"GO:0055120"},{"free_text":"alpha-actinin"},{"free_text":"Z-line component"}],"SOURCEOFCONTRAST":[{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"},{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"}],"MOLECULARFUNCTION":[{"onto_name":"actin binding","onto_id":"GO:0003779"},{"free_text":"actin cross-linking"}],"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":0.067},"axis":"X"}},{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":0.067},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Gallus gallus gallus","onto_id":"NCBITaxon:208526"},"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"detergent permeabilized","onto_id":"FBbi:00000262"}],"VISUALIZATIONMETHODS":{"onto_name":"Alexa Fluor 546","onto_id":"FBbi:00000441"},"CELLTYPE":[{"onto_name":"early embryonic cell","onto_id":"CL:0000007"},{"onto_name":"cardiac muscle cell","onto_id":"CL:0000746"}]}},"Citation":{"Title":"Caroline McKeown (2010) CIL:1584, Gallus gallus gallus, early embryonic cell, cardiac muscle cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil1584","DOI":"doi:10.7295\/W9CIL1584"}}}