{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1331182800},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"40376.zip","Size":13787264,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"40376.jpg","Size":122279,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Mp4","File_path":"40376_web.mp4","Size":754558,"Mime_type":"video\/mp4"}],"CORE":{"ATTRIBUTION":{"Contributors":["Nick Ferenz","Patricia Wadsworth"]},"BIOLOGICALPROCESS":[{"onto_name":"mitosis","onto_id":"GO:0007067"},{"onto_name":"mitotic metaphase","onto_id":"GO:0000089"},{"onto_name":"microtubule cytoskeleton organization","onto_id":"GO:0000226"}],"CELLLINE":{"onto_name":"LLC-PK1","onto_id":"MCC:0000286"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Time lapse movie of a porcine kidney LLCPK1 cell stably expressing photoactivable GFP-tubulin. Fluorescence was activated at metaphase in a vertical strip between the metaphase plate and spindle pole.  The activated strip moves poleward, demonstrating a marked microtubule flux during mitotic metaphase. The time series of images used to prepare the movie is included in this group of images."},"ITEMTYPE":{"onto_name":"charge coupled device (CCD)","onto_id":"FBbi:00000294"},"CELLULARCOMPONENT":{"onto_name":"microtubule cytoskeleton","onto_id":"GO:0015630"},"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"10944","TECHNICALDETAILS":{"free_text":"Cells were imaged using a Nikon Eclipse TE300 microscope with 100x phase NA 1.4 objective lens and spinning disk confocal head (Perkin Elmer) and images captured with a Hamamatsu Orca ER cooled CCD camera every 2 sec. Photoactivation was achieved with a 5-sec exposure to 413-nm light from an X-Cite 120 lamp.\nSee also: NP Ferenz and P Wadsworth 2007. Prophase microtubule arrays undergo flux-like behavior in mammalian cells. Mol Biol Cell 18:3993-4002"},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"spinning disk confocal microscopy","onto_id":"FBbi:00000253"},"DIMENSION":[{"Space":{"Image_size":672,"axis":"X"}},{"Space":{"Image_size":512,"axis":"Y"}},{"Time":{"unit":"seconds","value":2}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Sus scrofa domestica","onto_id":"NCBITaxon:9825"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"EGFP","onto_id":"FBbi:00000082"},"CELLTYPE":{"onto_name":"kidney epithelial cell","onto_id":"CL:0002518"}}},"Citation":{"Title":"Nick Ferenz, Patricia Wadsworth (2012) CIL:40376, Sus scrofa domestica, kidney epithelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil40376","DOI":"doi:10.7295\/W9CIL40376"}}}