{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1308024000},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"35211.tif","Size":984200000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"35211.jpg","Size":164115,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"35211.zip","Size":983111527,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"Journal Article","Href":"http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC2924811\/"}],"PUBLISHED":["Cell Physiol Biochem 2010;25:279-292"],"Contributors":["Søren T. Christensen","Linda Schneider","Michael Cammer"],"PUBMED":["20110689"]},"BIOLOGICALPROCESS":{"onto_name":"cell motility","onto_id":"GO:0048870"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"free_text":"MEF"},"PARAMETERIMAGED":{"onto_name":"elastic scattering of photons","onto_id":"FBbi:00000587"},"IMAGEDESCRIPTION":{"free_text":"Time lapse movie of mouse embryonic fibroblasts in culture imaged at 30 second intervals by phase contrast microscopy.  A micropipette is positioned near the cells to deliver either a growth factor or control buffer.  In this sequence buffer is added."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"ruffle","onto_id":"GO:0001726"},"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"differences in amount of elastic light scattering","onto_id":"FBbi:00000600"},"GROUP_ID":"8539","MOLECULARFUNCTION":{"onto_name":"receptor signaling protein tyrosine kinase activity","onto_id":"GO:0004716"},"TECHNICALDETAILS":{"free_text":"These data were collected as research which led to the published article Cell Physiol Biochem 2010;25:279-292.  Microscopy was performed with a 10X planapo phase contrast objective on an Olympus IX 70 microscope with a SensicamHQ cooled CCD camera and a heated stage insert set to 37 degrees C.  Cells were grown and serum starved for 48 h in a coverslip bottom MatTek dish. Small amounts of PBS were continuously ejected from the micropipette  to create a gradient in the vicinity of growth-arrested Tg737orpk MEFs. Cell movement was monitored with time lapse video microscopy, taking images at 30 second intervals.  A Femtojet Micromanipulator 5171 (Eppendorf-Brinkman Instruments) and a pump (model Femtojet; Eppendorf) were used to control the position of the micropipette and the pressure\nrequired for the chemoattractant flow. Femptotip II micropipettes were positioned within 1 μm\nof the cover slip and pressure set at 30 to 45 hPa. Spatial scale is approximate.\nImaging performed at the Image Analysis Facility of the Albert Einstein College of Medicine."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"phase contrast microscopy","onto_id":"FBbi:00000247"},"DIMENSION":[{"Space":{"Image_size":1280,"Pixel_size":{"unit":"microns","value":0.33},"axis":"X"}},{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":0.33},"axis":"Y"}},{"Time":{"unit":"seconds","value":30,"frame":"375"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"visualization of contiguous regions","onto_id":"FBbi:00000396"},"CELLTYPE":{"onto_name":"fibroblast","onto_id":"CL:0000057"}}},"Citation":{"Title":"Søren T. Christensen, Linda Schneider, Michael Cammer (2011) CIL:35211, Mus musculus, fibroblast. CIL. Dataset","ARK":"ark:\/b7295\/w9cil35211","DOI":"doi:10.7295\/W9CIL35211"}}}