{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1415595600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"43759.tif","Size":300000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"43759.jpg","Size":10595,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"43759.zip","Size":270476,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"OTHER":["Ensembl ID ENSG00000138778"],"Contributors":["Zoltan Maliga"]},"BIOLOGICALPROCESS":{"onto_name":"nuclear division","onto_id":"GO:0000280"},"CELLLINE":{"onto_name":"HeLa","onto_id":"MCC:0000219"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Shown is a fluorescence microscopy image of a  HeLa cell at mitotic metaphase. The cell is stained to reveal DNA (blue), microtubules (red), and the C-terminal portion of the KIF2A protein (green) which is largely confined to the compact chromosomes.\nThe image is part of a large project to characterize the locations of recombinantly tagged kinesin and myosin genes expressed from human or mouse-derived bacterial artificial chromosomes (BACs) as a resource for cell biology research.  \nSee also: Maliga et al. 2013 A genomic toolkit to investigate kinesin and myosin motor funtion in cells. Nature Cell Biol; published online Feb 2013;DCI:10.1038\/ncb2689."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"condensed chromosome","onto_id":"GO:0000793"},{"onto_name":"mitotic spindle","onto_id":"GO:0072686"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":[{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"}],"GROUP_ID":"16954","TECHNICALDETAILS":{"free_text":"HeLa cells carrying the appropriate EGFP-labeled transgene were fixed in cold methanol, rehydrated, and treated with mouse antibody to alpha-tubulin and goat antibody to GFP followed by donkey-anti-mouse Alexa 594, donkey-anti-goat Alexa 488 and Hoechst 33342. Preparations were examined using a Zeiss Axioplan 2 widefield microscope with a 63x NA 1.4 objective lens. Images were recorded using a CCD camera (CS472-95, Hamamatsu). \nFor further details see: Zaliga M et al. 2013 A genomic toolkit to investigate kinesin and myosin motor funtion in cells. Nat. Cell Biol. published online Feb 2013;DCI:10.1038\/ncb2689."},"DATAQUALIFICATION":{"free_text":"PROCESSED;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"attribution_cc_by"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":300,"Pixel_size":{"unit":"microns","value":0.106},"axis":"X"}},{"Space":{"Image_size":300,"Pixel_size":{"unit":"microns","value":0.106},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":{"onto_name":"methanol fixed tissue","onto_id":"FBbi:00000007"},"VISUALIZATIONMETHODS":[{"onto_name":"Hoechst 33342","onto_id":"FBbi:00000052"},{"onto_name":"EGFP","onto_id":"FBbi:00000082"},{"onto_name":"Alexa Fluor 594","onto_id":"FBbi:00000444"}],"CELLTYPE":{"onto_name":"epithelial cell","onto_id":"CL:0000066"}}},"Citation":{"Title":"Zoltan Maliga (2014) CIL:43759, Homo sapiens, epithelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil43759","DOI":"doi:10.7295\/W9CIL43759"}}}