{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1314331200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"187.jpg","Size":1258576,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Jpeg","File_path":"187_orig.jpg","Size":2779653,"Mime_type":"image\/jpeg"},{"File_type":"OME_tif","File_path":"187.tif","Size":38500000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"187.zip","Size":2779783,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Hermann Steller"]},"BIOLOGICALPROCESS":{"onto_name":"spermatogenesis","onto_id":"GO:0007283"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"The image shows a developing Drosophila spermatid immunostained for caspase activity (green). TRITC-phalloidin is used to label actin (red).  Caspase is required in spermiogenesis for the elimination of unwanted organelles and cytoplasm via apoptosis."},"ITEMTYPE":{"onto_name":"charge coupled device (CCD)","onto_id":"FBbi:00000294"},"CELLULARCOMPONENT":{"free_text":"cytoplasm"},"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"},"TECHNICALDETAILS":{"free_text":"See E. Arama et al. 2003 Caspase activity and a specific cytochrome C are required for sperm differentiation in Drosophila. Dev. Cell 4:687-697."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":3200,"axis":"X"}},{"Space":{"Image_size":4000,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Drosophila melanogaster","onto_id":"NCBITaxon:7227"},"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"ethanol fixed tissue","onto_id":"FBbi:00000006"}],"VISUALIZATIONMETHODS":{"onto_name":"Tetramethyl rhodamine (TRITC)","onto_id":"FBbi:00000454"},"CELLTYPE":{"onto_name":"spermatid","onto_id":"CL:0000018"}}},"Citation":{"Title":"Hermann Steller (2011) CIL:187, Drosophila melanogaster, spermatid. CIL. Dataset","ARK":"ark:\/b7295\/w9cil187","DOI":"doi:10.7295\/W9CIL187"}}}