{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1303099200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"13707.zip","Size":529275,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"13707.tif","Size":600000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"13707.jpg","Size":35085,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/3404\/"}],"PUBLISHED":["J Cell Biol. 191: 933-942, 2010."],"Contributors":["Seok Min Jin","Michael Lazarou","Chunxin Wang","Lesley A. Kane","Derek P. Narendra","Richard J. Youle"],"PUBMED":["21115803"]},"BIOLOGICALPROCESS":[{"onto_name":"mitochondrion degradation","onto_id":"GO:0000422"},{"onto_name":"protein K48-linked ubiquitination","onto_id":"GO:0070936"},{"onto_name":"protein K63-linked ubiquitination","onto_id":"GO:0070534"},{"onto_name":"protein ubiquitination involved in ubiquitin-dependent protein catabolic process","onto_id":"GO:0042787"}],"PROCESSINGHISTORY":{"free_text":"brightness and contrast adjusted"},"CELLLINE":{"onto_name":"HeLa","onto_id":"MCC:0000219"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"YFP-Parkin (green) localizes to mitochondria (red) when HeLa cells are transfected with control siRNA (siCtrl) and treated with the mitochondrial depolarizing agent CCCP (carbonyl cyanide-m-chlorophenyl hydrazone). HeLa cells stably expressing YFP-Parkin were transfected with control siRNA for 192 hours, treated with CCCP (10µM) for 1 hour and then stained with TMRE, a cationic fluorescent dye that is readily sequestered by active mitochondria. Cells were imaged using an inverted microscope (LSM510 Meta; Carl Zeiss, Inc.) with a 63× 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the accompanying image browser (LSM; Carl Zeiss, Inc.). Image corresponds to Fig1f, middle panels and is a control for other images in Fig1f in J Cell Biol. 191: 933-942, 2010. Images in Fig1f include CIL#s 13707, 13708, 13709."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"mitochondrion","onto_id":"GO:0005739"},{"onto_name":"cytosol","onto_id":"GO:0005829"},{"onto_name":"nucleus","onto_id":"GO:0005634"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":[{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},{"onto_name":"compartmentalization of stain or label","onto_id":"FBbi:00000604"}],"GROUP_ID":"7051","MOLECULARFUNCTION":[{"onto_name":"ubiquitin-protein ligase activity","onto_id":"GO:0004842"},{"onto_name":"zinc ion binding","onto_id":"GO:0008270"},{"onto_name":"protein binding","onto_id":"GO:0005515"},{"onto_name":"oxidative phosphorylation uncoupler activity","onto_id":"GO:0017077"}],"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"single-spot confocal microscopy","onto_id":"FBbi:00000252"},"DIMENSION":[{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.1395},"axis":"X"}},{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.1395},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":[{"onto_name":"EYFP","onto_id":"FBbi:00000084"},{"onto_name":"probe for mitochondria","onto_id":"FBbi:00000411"},{"free_text":"tetramethylrhodamine, ethyl ester (TMRE)"}],"CELLTYPE":[{"onto_name":"epithelial cell","onto_id":"CL:0000066"},{"free_text":"cervical carcinoma"}]}},"Citation":{"Title":"Seok Min Jin, Michael Lazarou, Chunxin Wang, Lesley A. Kane, Derek P. Narendra, Richard J. Youle (2011) CIL:13707, Homo sapiens, epithelial cell, cervical carcinoma. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13707","DOI":"doi:10.7295\/W9CIL13707"}}}