Raviola, Giuseppina; Elias, Peter; Friend, Daniel (2021). CIL:11215, Mus musculus, non-pigmented ciliary epithelial cell, epidermal cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0JH3K0R

Images of desmosomes from conventional EM and freeze fracture preparations show transmembrane filaments that link two hemidesmosomes. Upper: section of a cell boundary in the ciliary epithelium of the eye; middle, cross fracture across the boundary between two ciliary epithelial cells; lower, replica of a portion of the plasma membrane of an epidermal cell from newborn mouse. Figures 90 (upper), 91 (middle) both courtesy of Giuseppina Raviola, and 92 (lower, from Peter Elias and Daniel Friend) from Chapter 3 (Junctional Specializations) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.

• 2021

• Elias, Peter
• Friend, Daniel
• Raviola, Giuseppina

Preparation: crosslinking-fixative fixed tissue; tissue in plastic embedment; freeze_fracture/freeze_etch
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: stain with broad specificity; differences in deposition of metal shadow
Visualization methods: osmium tetroxide; shadowing and plating
Data qualification: Processed

• Cell Image Library Group ID: 11071

• Mus musculus

• Desmosome
• Epidermal cell
• Intermediate filament
• Keratin filament
• Non-pigmented ciliary epithelial cell
• Plasma membrane

• Cell adhesion
• Cell-cell junction organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 11215

Doi: https://doi.org/10.6075/J0JH3K0R

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL11215

Other resource

• Fawcett, Don W. "The Cell." 2nd ed., W.B. Saunders, 1981, ISBN 0721635849. https://www.ascb.org/career-development/teaching/don-fawcetts-the-cell/

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2022-08-12