{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1307073600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"24808.tif","Size":12700000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"24808.zip","Size":12622118,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"24808.jpg","Size":3052709,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["J Cell Biol. 1999 May 31;145(5):1009-26."],"Contributors":["Tatyana M. Svitkina","Gary G. Borisy"],"PUBMED":["10352018"]},"BIOLOGICALPROCESS":[{"onto_name":"cellular localization","onto_id":"GO:0051641"},{"onto_name":"actin filament organization","onto_id":"GO:0007015"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"optical path length gradient","onto_id":"FBbi:00000312"},"IMAGEDESCRIPTION":{"free_text":"Localization of XAC (Xenopus ADF\/cofilin) in Xenopus keratocytes done with immuno-EM.  A low mag view of the cell from which this high mag view is taken is shown in CIL 24807.  Image corresponds to Figure 8h from J Cell Biol. 1999 May 31;145(5):1009-26.  A fluorescence image showing XAC and phalloidin labeling is available as CIL 24807."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"lamellipodium","onto_id":"GO:0030027"},{"onto_name":"actin cytoskeleton","onto_id":"GO:0015629"},{"free_text":"lamella"},{"free_text":"ADF\/Cofilin"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"differences in deposition of metal shadow","onto_id":"FBbi:00000601"},"GROUP_ID":"9875","MOLECULARFUNCTION":{"onto_name":"structural constituent of cytoskeleton","onto_id":"GO:0005200"},"TECHNICALDETAILS":{"free_text":"Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998)."},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":4014,"axis":"X"}},{"Space":{"Image_size":3141,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Xenopus laevis","onto_id":"NCBITaxon:8355"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"permeabilized tissue","onto_id":"FBbi:00000093"},{"free_text":"polyethelyene glycol"}],"VISUALIZATIONMETHODS":[{"onto_name":"shadowing and plating","onto_id":"FBbi:00000398"},{"free_text":"platinum replica"},{"free_text":"immuno-gold"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"}],"CELLTYPE":{"free_text":"keratocyte"}}},"Citation":{"Title":"Tatyana M. Svitkina, Gary G. Borisy (2011) CIL:24808, Xenopus laevis, keratocyte. CIL. Dataset","ARK":"ark:\/b7295\/w9cil24808","DOI":"doi:10.7295\/W9CIL24808"}}}