Lauzon, Dee; Lancelle, Sue; Rice, Marian (2021). CIL:39765, Mus musculus, cardiac muscle cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J01J9929

Transmission electron micrograph of mouse atrial cardiac muscle showing a close up image of the intercalated discs, which support synchronized contraction of the cardiac muscle.

• 2021

• Lancelle, Sue
• Lauzon, Dee
• Rice, Marian

Primary fixation included: 2.5 % glutaraldehyde, 2% formaldehyde in 0.1 M Na-phosphate buffer, pH 7.4. Post-fixed in 2% OSO4 in 0.1 M Na-phosphate buffer, pH 7.4. Stained en bloc in 1% uranyl acetate. The tissue was then dehydrated in a graded series of ethanol and infiltrated with Spurr's resin. Thin sections of 70 nm were trimmed using a diamond knife and post-stained in uranyl acetate and lead citrate. This micrograph image was taken using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80kV.

Preparation: glutaraldehyde fixed tissue; formaldehyde fixed tissue; tissue in epoxy resin embedment; ethanol dehydration
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; lead salt
Processing history: unprocessed raw data

• Cell Image Library Group ID: 10969

• Mus musculus

• Cardiac muscle cell
• Intercalated disc

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39765

Doi: https://doi.org/10.6075/J01J9929

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39765

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2022-08-12