{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1313380800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"130.tif","Size":10300000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"130.zip","Size":6463758,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"130.jpg","Size":1758880,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/www5.pbrc.hawaii.edu\/allen\/"}],"Contributors":["Richard Allen (University of Hawaii)"]},"BIOLOGICALPROCESS":{"onto_name":"contractile vacuole organization","onto_id":"GO:0033298"},"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"free_text":"Print from negative scanned for Photoshop."}],"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"The contractile vacuole pore is coated on its cytosolic side by helically wound microtubules. Other microtubules abut this helix and extend out to the surface of the contractile vacuole where they are attached to the CV membrane and hold the contractile vacuole in place. The plasma membrane and CV membrane are separated only by a narrow gap at the bottom of the pore. TEM taken on 2\/11\/71 by R. Allen with Hitachi HU11A operating at 60kV. Neg. 19,500X. Bar = 0.2µm. There is a high resolution version of this image in the library (CIL:39793) which is available for quantitative analysis. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"contractile vacuole","onto_id":"GO:0000331"},{"onto_name":"contractile vacuole pore","onto_id":"GO:0031913"},{"onto_name":"contractile vacuolar membrane","onto_id":"GO:0031164"},{"onto_name":"cell cortex","onto_id":"GO:0005938"},{"onto_name":"microtubule associated complex","onto_id":"GO:0005875"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"10770","TECHNICALDETAILS":{"free_text":"Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":3213,"axis":"X"}},{"Space":{"Image_size":3198,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Tetrahymena pyriformis","onto_id":"NCBITaxon:5908"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (University of Hawaii) (2011) CIL:130, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil130","DOI":"doi:10.7295\/W9CIL130"}}}