{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1329195600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"39791.zip","Size":72849,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"39791.jpg","Size":56484,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Jpeg","File_path":"39791_orig.jpg","Size":72711,"Mime_type":"image\/jpeg"},{"File_type":"OME_tif","File_path":"39791.tif","Size":1000000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"Dartmouth EM Facility","Href":"http:\/\/remf.dartmouth.edu\/images\/SeaUrchinSEM\/source\/10.html"}],"Contributors":["Louisa Howard"]},"BIOLOGICALPROCESS":{"free_text":"embryonic morphogenesis"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"elastic scattering of electrons","onto_id":"FBbi:00000586"},"IMAGEDESCRIPTION":{"free_text":"Scanning electron microscope image of Strongylocentrotus drobachiensus [sea urchin] embryo at the late gastrula stage. This high magnification image of the embryo shows the primary mesenchyme syncytial ring, where it attaches to the outer epithelial layer (ectoderm layer). The fine fibers are part of the blastocoel matrix. The primary mesenchyme syncytial ring is the precursor to spicule formation (developing calcareous larval skeleton).  This image is part of a group of SEM images of sea urchin embryos at various stages of development.  The group is available as CIL:39782-39791."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"extracellular matrix","onto_id":"GO:0031012"},{"onto_name":"extracellular region","onto_id":"GO:0005576"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"differences in deposition of metal shadow","onto_id":"FBbi:00000601"},"GROUP_ID":"10724","TECHNICALDETAILS":{"free_text":"Images taken on a  JEOL 35C SEM.  \nProcessing protocol:\n1. 2% GTA in 75% MBL SW or FilteredSW \nFSW - fix for 1.5hrs. RT.\n2. Rinse with 0.1M NaCacodylate buffer pH 7.4\n3. 0.5% OSO4 in .075M NaCacodylate buffer pH 7.4 - 30' in the cold\n4. Rinse in 0.1M NaCacodylate buffer ph 7.4\n5. Dehydrate thru ETOH: 30%, 50%, 70%, 95% for 5 min each\n6. 100% - 3 changes - 15' each\n7. Critical Point Dry\n8. Stick embryos down onto silver paint. Gently crack open with glass needle (pipette drawn out to a fine point).\n9. Sputter coat with AuPd."},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"scanning electron microscopy (SEM)","onto_id":"FBbi:00000257"},"DIMENSION":[{"Space":{"Image_size":640,"axis":"X"}},{"Space":{"Image_size":480,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Lytechinus pictus","onto_id":"NCBITaxon:7653"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"free_text":"ethanol dehydration"},{"onto_name":"critical_point dried specimen","onto_id":"FBbi:00000581"}],"VISUALIZATIONMETHODS":[{"onto_name":"gold","onto_id":"FBbi:00000202"},{"onto_name":"visualization of contiguous regions","onto_id":"FBbi:00000396"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"}],"CELLTYPE":[{"onto_name":"mesenchymal cell","onto_id":"CL:0000134"},{"onto_name":"epithelial cell","onto_id":"CL:0000066"}]}},"Citation":{"Title":"Louisa Howard (2012) CIL:39791, Lytechinus pictus, mesenchymal cell, epithelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil39791","DOI":"doi:10.7295\/W9CIL39791"}}}