{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1280980800},"Data_type":{"Still_image":false,"Z_stack":false,"Video":false,"Time_series":true},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"730.zip","Size":93481544,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"730.jpg","Size":24344,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"730.tif","Size":78800000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"Contributors":["George H. Patterson"]},"BIOLOGICALPROCESS":{"onto_name":"peroxisome organization","onto_id":"GO:0007031"},"CELLLINE":{"onto_name":"COS-7","onto_id":"MCC:0000112"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"COS 7 cell expressing Organelle LightsTM Peroxi-GFP (Invitrogen)consisting of the peroxisomal C-terminal targeting sequence. Imageswere collected with a 63X 1.4 NA Plan-Apochromat oil objective on a\nZeiss LSM510 META (Carl Zeiss, Thornwood, NY) using 488 nm excitation, HFT 488 dichroic, and LP505 emission filter. Image size is 512 x 512,\npixel size is 0.14 µm x 0.14 µm and pixel time was set to 1.6 µsec. Detector pinhole was set to collect 300 ~1 µm slices at ~1 sec intervals"},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"peroxisome","onto_id":"GO:0005777"},"RELATIONTOINTACTCELL":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"SOURCEOFCONTRAST":{"onto_name":"EGFP","onto_id":"FBbi:00000082"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"single-spot confocal microscopy","onto_id":"FBbi:00000252"},"DIMENSION":[{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.1428},"axis":"X"}},{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.1428},"axis":"Y"}},{"Time":{"unit":"seconds","value":1,"frame":"100"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Chlorocebus sabaeus","onto_id":"NCBITaxon:60711"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"UV\/visible\/IR photons","onto_id":"FBbi:00000378"}}},"Citation":{"Title":"George H. Patterson (2010) CIL:730, Chlorocebus sabaeus. CIL. Dataset","ARK":"ark:\/b7295\/w9cil730","DOI":"doi:10.7295\/W9CIL730"}}}