{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1305432000},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"21761.tif","Size":800000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"21761.zip","Size":264456,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"21761.jpg","Size":20002,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/www.broadinstitute.org\/bbbc\/drosophila_kc167_1.html"}],"Contributors":["Anne Carpenter"]},"BIOLOGICALPROCESS":{"free_text":"dsRNA Anillin treatement"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"free_text":"Kc167"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Five different samples of Drosophila melanogaster Kc167 cells were stained with Hoechst 33342, a DNA stain. The last sample (labeled nodsRNA, CIL 21789) is of wild-type cells. Each of the other four samples (labeled 48, 340, Anillin, and mad2, CIL 21749, 21739, 21759, and 21773, respectively) has a different gene knocked down by RNAi.  This is the set that were treated with Anillin RNAi.  There are 10 images of each sample, for a total of 50 images. The images were acquired on a Zeiss Axiovert 200M microscope. A recommended use for these five image sets is to algorithmically compute the number of cells in each image.  A tab-delimited text file containing this information can be found at the URL indicated in the attribution box.  The recommended citation when using these images is:  \"We used the Drosophila Kc167 1 image set (Carpenter, et al., Genome Biology, 2006) available from the Broad Bioimage Benchmark Collection (www.broad.mit.edu\/bbbc).\""},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"free_text":"DNA"},"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"free_text":"distribution of DNA"},"GROUP_ID":"8119","TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":512,"axis":"X"}},{"Space":{"Image_size":512,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Drosophila melanogaster","onto_id":"NCBITaxon:7227"},"VISUALIZATIONMETHODS":{"onto_name":"Hoechst 33342","onto_id":"FBbi:00000052"},"CELLTYPE":{"free_text":"epithelial"}}},"Citation":{"Title":"Anne Carpenter (2011) CIL:21761, Drosophila melanogaster, epithelial. CIL. Dataset","ARK":"ark:\/b7295\/w9cil21761","DOI":"doi:10.7295\/W9CIL21761"}}}