{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1290402000},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"9714.zip","Size":14986494,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"9714.tif","Size":7400000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"9714.jpg","Size":1195913,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"Contributors":["Richard Allen"]},"BIOLOGICALPROCESS":[{"onto_name":"macronucleus organization","onto_id":"GO:0032124"},{"onto_name":"nuclear pore organization","onto_id":"GO:0006999"},{"onto_name":"chromatin organization","onto_id":"GO:0006325"},{"onto_name":"chromosome organization","onto_id":"GO:0051276"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"free_text":"Print from negative scanned for Photoshop."}],"CELLLINE":{"free_text":"Cambridge Culture Collection, Cambridge, England, catalog # LB1656\/1"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"High resolution image of the macronucleus of Coleps hirtus. Heterochromatin and nucleoli are seen as smaller and larger stained bodies, respectively, in the macronucleus. Many nuclear pores exist in the nuclear envelope."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"macronucleus","onto_id":"GO:0031039"},{"onto_name":"nuclear pore","onto_id":"GO:0005643"},{"onto_name":"heterochromatin","onto_id":"GO:0000792"},{"onto_name":"nucleolus","onto_id":"GO:0005730"}],"SOURCEOFCONTRAST":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"GROUP_ID":"10163","MOLECULARFUNCTION":[{"onto_name":"structural constituent of chromatin","onto_id":"GO:0030527"},{"onto_name":"structural constituent of nuclear pore","onto_id":"GO:0017056"}],"TECHNICALDETAILS":{"free_text":"The material was prepared by standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 5\/23\/69 by R. Allen with Philips 300 operating at 60kV. Neg. 7,650X.  The raw film was scanned with a Nikon Coolscan 9000ED. This high resolution image is suitable for quantitative analysis. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"DATAQUALIFICATION":{"free_text":"PROCESSED;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":3000,"axis":"X"}},{"Space":{"Image_size":2432,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Coleps hirtus","onto_id":"NCBITaxon:60000"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":[{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (2010) CIL:9714, Coleps hirtus, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil9714","DOI":"doi:10.7295\/W9CIL9714"}}}