{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1291266000},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"10010.zip","Size":19625314,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"10010.tif","Size":10300000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"10010.jpg","Size":2579118,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/www5.pbrc.hawaii.edu\/allen\/"}],"Contributors":["Richard Allen"]},"BIOLOGICALPROCESS":[{"onto_name":"feeding behavior","onto_id":"GO:0007631"},{"onto_name":"exocyst assembly","onto_id":"GO:0001927"},{"onto_name":"killing of cells of other organism","onto_id":"GO:0031640"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"free_text":"Print from negative scanned for Photoshop."}],"CELLLINE":{"free_text":"Carolina Biological Supply Company, NC, U.S.A."},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Toxicysts, extrusive organelles, (sometimes called extrusomes in protistan lingo) in the proter (or anterior daughter) of a dividing cell. Toxicysts can be very long and are aligned along microtubular lamellae that extend from the cytopharynx. Extra myelin-like membrane can be seen bordering two of the three toxicysts shown. At the upper right is the fibrous cortex of Didinium with mucocysts, an extrusive organelle involved when cyst formation occurs. A digesting food vacuole is at the lower left. TEM taken on 5\/20\/69 by R. Allen with Philips 300 operating at 60kV. Neg. 6,370X. Bar = 1.0µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. A raw, unprocessed, high resolution versions of this image (CIL:4667 & 7313) are in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness.\n\nAdditional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"free_text":"toxicyst"},{"onto_name":"microtubule associated complex","onto_id":"GO:0005875"},{"onto_name":"cell cortex","onto_id":"GO:0005938"},{"free_text":"mucocyst"},{"onto_name":"food vacuole","onto_id":"GO:0020020"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"3360","DATAQUALIFICATION":{"free_text":"PROCESSED;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":3638,"axis":"X"}},{"Space":{"Image_size":2816,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Didinium nasutum","onto_id":"NCBITaxon:5997"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (2010) CIL:10010, Didinium nasutum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil10010","DOI":"doi:10.7295\/W9CIL10010"}}}