Ris, Hans (2021). CIL:41698, Cricetulus griseus, ovary. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0JW8D7H

High Voltage EM image of CHO metaphase chromosomes from a 'thick' section of metaphase-arrested cells treated with D20 to stabilize microtubules. This image was recorded with a specimen tilt of 55 degrees. The accompanying grouped image had a tilt of 45 degrees to create a stereo pair providing an oblique 3D view of the chromosomes.

• 1977-06-25

• 2021

• Ris, Hans

CHO cells at metaphase were treated with 50% D2O, fixed with glutaraldehyde, embedded in epoxy resin and 0.25 micrometer 'thick' sections stained with uranyl acetate and lead citrate for HVEM observation at 1MeV. See also: H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.

Preparation: chemically fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 12048

• Cricetulus griseus

• Nuclear chromosome
• Ovary

• CHO
• Chromosome organization
• Mitosis
• Mitotic metaphase

• data
• image

• No linguistic content; Not applicable

Samplenumber: 41698

Doi: https://doi.org/10.6075/J0JW8D7H

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL41698

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2023-08-17