{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1291266000},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"4664.zip","Size":40903816,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"4664.tif","Size":40800000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"4664.jpg","Size":8758859,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"Contributors":["Richard Allen"]},"BIOLOGICALPROCESS":[{"onto_name":"microtubule cytoskeleton organization","onto_id":"GO:0000226"},{"onto_name":"ciliary cell motility","onto_id":"GO:0060285"},{"onto_name":"detection of symbiotic bacterium","onto_id":"GO:0009604"}],"PROCESSINGHISTORY":[{"onto_name":"film","onto_id":"FBbi:00000303"},{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"}],"CELLLINE":{"free_text":"Carolina Biological Supply Company, NC, U.S.A."},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"A high resolution image Didinium. This ciliate has two bands of ciliated basal bodies that encircle the cell, one adorally and one aborally. The bands are composed of groups of approximately 15 ciliated basal bodies. This section is a longitudinal section of the cilium and basal body and cross section of approximately seven different groups or pectinelles. The proximal end of basal bodies are ensheathed in electron opaque sleeves from which microtubular ribbons extend into the adjacent ridges on the two sides of each basal body. TEM taken on 5\/20\/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X. The raw film was scanned with a Nikon Coolscan 9000ED. This image is available for quantitative analysis.\n\nStandard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness.\n\nAdditional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"cell cortex","onto_id":"GO:0005938"},{"onto_name":"cilium","onto_id":"GO:0005929"},{"onto_name":"microtubule basal body","onto_id":"GO:0005932"},{"onto_name":"ciliary rootlet","onto_id":"GO:0035253"},{"onto_name":"axoneme","onto_id":"GO:0005930"},{"free_text":"ectosymbiotic bacteria"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"3865","DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":5516,"Pixel_size":{"unit":"nanometers","value":1},"axis":"X"}},{"Space":{"Image_size":3789,"Pixel_size":{"unit":"nanometers","value":1},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Didinium nasutum","onto_id":"NCBITaxon:5997"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":[{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (2010) CIL:4664, Didinium nasutum, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil4664","DOI":"doi:10.7295\/W9CIL4664"}}}