{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1331182800},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Mp4","File_path":"40269_web.mp4","Size":384898,"Mime_type":"video\/mp4"},{"File_type":"Zip","File_path":"40269.zip","Size":21577856,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"40269.jpg","Size":45975,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"Contributors":["Nasser Rusan","Patricia Wadsworth"]},"BIOLOGICALPROCESS":[{"onto_name":"mitosis","onto_id":"GO:0007067"},{"onto_name":"mitotic metaphase","onto_id":"GO:0000089"},{"onto_name":"microtubule cytoskeleton organization","onto_id":"GO:0000226"}],"CELLLINE":{"onto_name":"LLC-PK1","onto_id":"MCC:0000286"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Time lapse movie of a porcine kidney LLCPK1 cell stably expressing EGFP-alpha tubulin. The movie shows microtubule behavior from early prophase through metaphase."},"ITEMTYPE":{"onto_name":"charge coupled device (CCD)","onto_id":"FBbi:00000294"},"CELLULARCOMPONENT":{"onto_name":"microtubule cytoskeleton","onto_id":"GO:0015630"},"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"10946","TECHNICALDETAILS":{"free_text":"Cells were imaged using a Nikon Eclipse TE300 microscope with 100x phase NA 1.4 objective lens and spinning disk confocal head (Perkin Elmer) and images captured with a MicroMax cooled CCD camera.\nImages were captured every 2 sec and are displayed at 7.5 frames\/sec. \nSee also:N Rusan and P Wadsworth 2005. Centrosome fragments and microtubules are transported asymmetrically away from division plane in mitosis.J Cell Biol 168:21-28."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"spinning disk confocal microscopy","onto_id":"FBbi:00000253"},"DIMENSION":[{"Space":{"Image_size":510,"axis":"X"}},{"Space":{"Image_size":423,"axis":"Y"}},{"Time":{"unit":"seconds","value":2}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Sus scrofa domestica","onto_id":"NCBITaxon:9825"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"EGFP","onto_id":"FBbi:00000082"},"CELLTYPE":{"onto_name":"kidney epithelial cell","onto_id":"CL:0002518"}}},"Citation":{"Title":"Nasser Rusan, Patricia Wadsworth (2012) CIL:40269, Sus scrofa domestica, kidney epithelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil40269","DOI":"doi:10.7295\/W9CIL40269"}}}