{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1326776400},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"39309.tif","Size":29600000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"39309.jpg","Size":4696747,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"39309.zip","Size":29550094,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Richard Allen (University of Hawaii)"]},"BIOLOGICALPROCESS":[{"onto_name":"plasma membrane organization","onto_id":"GO:0007009"},{"onto_name":"cortical cytoskeleton organization","onto_id":"GO:0030865"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Cross sections of striated bands and infraciliary lattice arms. Filaments of the striated bands are less densely packed than the infraciliary lattice. Kinetodesmal fibers and postciliary microtubules are also cross sectioned and are seen to come to lie near each other in the lateral ridges. A linkage would provide additional strength to the kineties. The observer is looking at a cross section of the cell from the posterior toward the anterior of the cell. TEM taken on 6\/28\/69 by R. Allen with Philips 300 operating at 60kV. Neg. 36,500X."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"pellicle","onto_id":"GO:0020039"},{"onto_name":"cell cortex","onto_id":"GO:0005938"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"10397","TECHNICALDETAILS":{"free_text":"Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":3336,"Pixel_size":{"unit":"nanometers","value":0.55},"axis":"X"}},{"Space":{"Image_size":4428,"Pixel_size":{"unit":"nanometers","value":0.55},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Paramecium multimicronucleatum","onto_id":"NCBITaxon:44030"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"free_text":"lead"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (University of Hawaii) (2012) CIL:39309, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil39309","DOI":"doi:10.7295\/W9CIL39309"}}}