{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1287028800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"763.zip","Size":22287684,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"763.tif","Size":22100000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"763.jpg","Size":2718229,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"Contributors":["Rachel Horowitz-Scherer","Chris Woodcock"]},"BIOLOGICALPROCESS":{"onto_name":"reproduction","onto_id":"GO:0000003"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":[{"onto_name":"modes of electron microscopy","onto_id":"FBbi:00000256"},{"onto_name":"EELS imaging","onto_id":"FBbi:00000381"}],"IMAGEDESCRIPTION":{"free_text":"Upon electron bombardment, some elements, such as P, show an electron energy loss profile with a peak at a specific energy loss value. By collecting a series of images of a field at different energy losses, it is possible to compute the relative amount of the element at each pixel and map the element's distribution, a technique known as electron spectroscopic imaging (ESI) or electron filtered transmission EM (EFTEM).\nThe 4 images in this group were recorded from unstained <30 nm section with 5eV energy windows centered on 120, 104, 160 and 170 eV respectively using a Zeiss 902 energy filtered EM, making it possible to map the distribution of phosphorus (energy loss edge 160 eV) within the chromatin fibers. This image was recorded at 1o4 eV."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"chromatin","onto_id":"GO:0000785"},"RELATIONTOINTACTCELL":{"onto_name":"sectioned tissue","onto_id":"FBbi:00000026"},"SOURCEOFCONTRAST":{"free_text":"electron scattering"},"GROUP_ID":"8090","MOLECULARFUNCTION":{"free_text":"DNA compaction"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"microscopy with lenses","onto_id":"FBbi:00000343"},{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"}],"DIMENSION":[{"Space":{"Image_size":4011,"Pixel_size":{"unit":"microns","value":0.4},"axis":"X"}},{"Space":{"Image_size":2804,"Pixel_size":{"unit":"microns","value":0.4},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Patiria miniata","onto_id":"NCBITaxon:46514"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"free_text":"freeze substitution in Lowicryl"}],"VISUALIZATIONMETHODS":{"free_text":"electron energy loss window"},"CELLTYPE":{"onto_name":"sperm","onto_id":"CL:0000019"}}},"Citation":{"Title":"Rachel Horowitz-Scherer, Chris Woodcock (2010) CIL:763, Patiria miniata, sperm. CIL. Dataset","ARK":"ark:\/b7295\/w9cil763","DOI":"doi:10.7295\/W9CIL763"}}}