{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1290920400},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"7342.jpg","Size":6423269,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"7342.zip","Size":35627180,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"7342.tif","Size":35700000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"Contributors":["Richard Allen"]},"BIOLOGICALPROCESS":[{"free_text":"centrin fibers"},{"free_text":"fiber targeting of rough endoplasmic reticulum"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"}],"CELLLINE":{"free_text":"Cambridge Culture Collection, Cambridge, England, # LB1657\/1"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"High resolution image of a myoneme of Opercularia coarctata. Such fibrous bundles are known to be composed of centrin, a filament that contracts when exposed to calcium. The ER is closely associated with the myonemes and sends specialized decorated tubules onto the surface or into the fibrous bundle. The peroxisome typically has one luminal tubule and mitochondria have tubular cristae. TEM taken on 6\/13\/69 by R. Allen with Philips 300 operating at 60kV. Neg. 30,200X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis.\n\nStandard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness.\n\nAdditional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"cortical actin cytoskeleton","onto_id":"GO:0030864"},{"free_text":"centrin fibers"},{"onto_name":"rough endoplasmic reticulum","onto_id":"GO:0005791"},{"onto_name":"peroxisomal membrane","onto_id":"GO:0005778"},{"onto_name":"peroxisomal matrix","onto_id":"GO:0005782"},{"onto_name":"mitochondrial inner membrane","onto_id":"GO:0005743"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"3549","MOLECULARFUNCTION":[{"onto_name":"intracellular ligand-gated calcium channel activity","onto_id":"GO:0005218"},{"free_text":"centrin"}],"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":3886,"Pixel_size":{"unit":"nanometers","value":0.66},"axis":"X"}},{"Space":{"Image_size":4584,"Pixel_size":{"unit":"nanometers","value":0.66},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":[{"onto_name":"Opercularia [NCBITaxon:168247]","onto_id":"NCBITaxon:168247"},{"free_text":"Opercularia coarctata"}],"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (2010) CIL:7342, Opercularia [NCBITaxon:168247], Opercularia coarctata, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil7342","DOI":"doi:10.7295\/W9CIL7342"}}}