Horowitz-Scherer, Rachel; Woodcock, Christopher (2021). CIL:770, Patiria miniata, sperm. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0WQ032W

Phosphorus has a characteristic electron energy loss with an edge at 160 eV. The Zeiss 902 electron microscope permits images to be recorded at different energy losses which can be digitized and processed to yield a net phosphorus image. For nuclear chromatin, phosphorus is primarily located in DNA, allowing its distribution in chromatin fibers to be mapped. Electron energy loss (EELS) imaging is also known as electron spectroscopic imaging (ESI) and electron energy filtering TEM (EFTEM).
Starfish sperm chromatin, which has clearly defined chromatin fibers was fixed, embedded in Lowicryl at low temperature, thin sections prepared and imaged unstained. The 3 grouped images were recorded with a Zeiss 902 energy filtered TEM at 80KV using 5eV windows centered on energy loss values of 160 (this image), 120, and 104eV.

• 2021

• Horowitz-Scherer, Rachel
• Woodcock, Christopher

Preparation: glutaraldehyde fixed tissue; Low temperature embedding
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: modes of electron microscopy; EELS imaging
Source of contrast: inelastic scattering
Visualization methods: EELS window
Processing history: unprocessed raw data

• Cell Image Library Group ID: 7769

• Patiria miniata

• Nuclear chromatin
• Sperm

• DNA packaging
• Fertilization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 770

Doi: https://doi.org/10.6075/J0WQ032W

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL770

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2022-08-12