{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1301457600},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"13457.tif","Size":2200000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"13457.jpg","Size":20640,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"13457.zip","Size":2197375,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/1851\/"}],"PUBLISHED":["J Cell Biol. 187: 967-975. 2009"],"Contributors":["Christopher S. Wood","Karl R. Schmitz","Nicholas J. Bessman","Thanuja Gangi Setty","Kathryn M. Ferguson","Christopher G. Burd"],"PUBMED":["20026658"]},"BIOLOGICALPROCESS":[{"onto_name":"retrograde vesicle-mediated transport, Golgi to ER","onto_id":"GO:0006890"},{"onto_name":"protein localization in Golgi apparatus","onto_id":"GO:0034067"},{"onto_name":"protein transport","onto_id":"GO:0015031"}],"PROCESSINGHISTORY":{"onto_name":"constrained iterative deconvolution","onto_id":"FBbi:00000360"},"CELLLINE":{"free_text":"BY4742 vps74∆"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Intracellular localization of GFP-GOLPH3 R171A, R174A mutant in BY4742 vps74∆. GFP-GOLPH3 is localized to Golgi compartments and the cytosol (CIL# 13455) but loses localization when two amino acids are mutated in the sulfate-binding pocket. This study demonstrates that the sulfate-binding pocket of Vps74 and GOLPH3 mediates PtdIns4P-binding and is essential for function. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig4A GFP-GOLPH3 R171A R174A\/vps74∆ panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 4A include CIL#s 13449, 13451, 13453, 13455, 13456, 13457, 24816, 24817, 24818, 24819, 24820, 24821."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"Golgi apparatus","onto_id":"GO:0005794"},{"onto_name":"cytosol","onto_id":"GO:0005829"},{"onto_name":"extrinsic to membrane","onto_id":"GO:0019898"},{"onto_name":"nucleus","onto_id":"GO:0005634"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"6596","MOLECULARFUNCTION":[{"onto_name":"phosphatidylinositol-4-phosphate binding","onto_id":"GO:0070273"},{"onto_name":"enzyme binding","onto_id":"GO:0019899"}],"DATAQUALIFICATION":{"free_text":"PROCESSED;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":302,"Pixel_size":{"unit":"microns","value":0.0663},"axis":"X"}},{"Space":{"Image_size":302,"Pixel_size":{"unit":"microns","value":0.0663},"axis":"Y"}},{"Space":{"Image_size":12,"Pixel_size":{"unit":"microns","value":0.4},"axis":"Z"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Saccharomyces cerevisiae S288c","onto_id":"NCBITaxon:559292"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"EGFP","onto_id":"FBbi:00000082"}}},"Citation":{"Title":"Christopher S. Wood, Karl R. Schmitz, Nicholas J. Bessman, Thanuja Gangi Setty, Kathryn M. Ferguson, Christopher G. Burd (2011) CIL:13457, Saccharomyces cerevisiae S288c. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13457","DOI":"doi:10.7295\/W9CIL13457"}}}