{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1303531200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"27228.zip","Size":6258262,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"27228.tif","Size":6300000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"27228.jpg","Size":1064370,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"Contributors":["Wai Yan Lam","Marian Rice"]},"BIOLOGICALPROCESS":{"onto_name":"cytoskeleton organization","onto_id":"GO:0007010"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Rough endoplasmic reticulum and Golgi in the epidermal cell of Fundulus heteroclitus scale. Endoplasmic reticulum studded with protein-manufacturing ribosomes form a continuous network along the periphery of this section of the cell. Along the surface of some of the ER folds are energy-generating mitochondria. Deeper into the cell are stacks of cisternae forming the Golgi. Vesicles packaged by the Golgi are visible at the peripheries of these stacks and are found scattered throughout the cytoplasm."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"rough endoplasmic reticulum","onto_id":"GO:0005791"},{"onto_name":"Golgi apparatus","onto_id":"GO:0005794"},{"onto_name":"ribosome","onto_id":"GO:0005840"},{"onto_name":"mitochondrion","onto_id":"GO:0005739"},{"free_text":"vesicles"}],"RELATIONTOINTACTCELL":{"onto_name":"sectioned tissue","onto_id":"FBbi:00000026"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"GROUP_ID":"9597","TECHNICALDETAILS":{"free_text":"Fundulus heteroclitus scales were chemically fixed with 2.5% glutaraldehyde, 2% formaldehyde in 0.1M cacodylate buffer (pH 7.3), then post-fixed in 4% osmium tetroxide and stained en bloc in 1% uranyl acetate. The scales were then dehydrated in a graded series of ethanol and infiltrated with Spurr\u2019s resin. Thin sections of 70 nm were trimmed using a diamond knife and post-stained in uranyl acetate and lead citrate. This micrograph was imaged using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80 kV."},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":1715,"Pixel_size":{"unit":"nanometers","value":1.92},"axis":"X"}},{"Space":{"Image_size":1815,"Pixel_size":{"unit":"nanometers","value":1.92},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Fundulus heteroclitus","onto_id":"NCBITaxon:8078"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"free_text":"dehydrated in ethanol"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"}],"VISUALIZATIONMETHODS":[{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":{"onto_name":"epidermal cell","onto_id":"CL:0000362"}}},"Citation":{"Title":"Wai Yan Lam, Marian Rice (2011) CIL:27228, Fundulus heteroclitus, epidermal cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil27228","DOI":"doi:10.7295\/W9CIL27228"}}}