3699
2007-04-01 00:00:00.0
Tom Deerinck
Guido Gaietta
purpose
Tomographic reconstruction of caulobacter
a .mpg format movie file showing the aligned tilt series taken on an IVEM, shot onto film.
/telescience/home/CCDB_DATA_USER.portal/P1220/Experiment_3439/Subject_174/Tissue_205/Microscopy_3699/cb0_tilt.mpg
A Caulobacter crescentus specimen taken at a 0 degree tilt. Gold fiducials are 10 and 15 nm in diameter.
<![CDATA[http://ccdb.ucsd.edu/sand/main?event=rd&type=512r&mpid=3699]]>
A .tar file containing all processing files for an IMOD volume
reconstruction. Along with necessary .st, .preali, .ali and .rawtlt files
is a notes.txt file containing some information necessary for proper
processing.
/telescience/home/CCDB_DATA_USER.portal/P1220/Experiment_3439/Subject_174/Tissue_205/Microscopy_3699/cb0_img.tar
30000
film
60 degrees
-60 degrees
10 and 15 nm gold fiducials
singlet_desc
2 degrees
2007-04-25 00:00:00.0
3699
cb0
JEOL 4000 #1
IVEM
SINGLE TILT
4482
6678
Patrick H. Viollier
Tom Deerinck
Mason Mackey
John Crum
Mark Ellisman
Department of Energy
Lucy Shapiro
Harley McAdams
Caulobacter
2003-07-01 00:00:00.0
P1220
A .mpg file movie of the slice by slice reconstructed volume of a caulobacter crescentus specimen.
/telescience/home/CCDB_DATA_USER.portal/P1220/Experiment_3439/Subject_174/Tissue_205/Microscopy_3699/cb0_slicebyslice.mpg
A maximum intensity projection image of a caulobacter crescentus specimen taken on an IVEM.
<![CDATA[http://ccdb.ucsd.edu/sand/main?event=rd&type=512v&mpid=3699]]>
A .tar file containing to .mrc format slice by slice volume
reconstructions. One is a trimmed stack of the other larger volume. Both
files were made using IMOD's tomographic reconstruction program.
/telescience/home/CCDB_DATA_USER.portal/P1220/Experiment_3439/Subject_174/Tissue_205/Microscopy_3699/cb0_vol.tar
1156,3116,1269
Caulobacter crescentus
Induced for 1 hour at 30 degrees CLabeled in M2G medium for 2 hours at 30 degrees C (in the presence of 1.8 micromolar ReAsH-EDT2) and photoconvertedSamples were fixed with 2.5% glutaraldehyde with 1% acrolein in 0.05M sodium cacodylate pH 7.4 with 3 mM CaCl2 and 1 % sucrose followed by conventional osmium tetroxide/potassium ferrocyanide post-fixation, dehydration and embedding.
conventional fixation
N/A
male
Caulobacter crescentus (Snc04)
Caulobacter crescentus
Snc04
N/A
.4 um
N/A