{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1313640000},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"36768.jpg","Size":2403960,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"36768.tif","Size":10400000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"36768.zip","Size":20652750,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Richard Allen (University of Hawaii)"]},"BIOLOGICALPROCESS":[{"onto_name":"oral apparatus organization","onto_id":"GO:0032122"},{"onto_name":"ciliary or flagellar motility","onto_id":"GO:0001539"},{"onto_name":"endocytosis","onto_id":"GO:0006897"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"free_text":"Print from negative scanned for Photoshop."}],"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"The highest concentration of parasomal sacs is found in the oral region associated with the three ciliary membranelles, called in Paramecium, the ventral amd dorsal peniculi and the quadrulus. This micrograph shows the four rows each of the two peniculi, dorsal and ventral, and a large accumulation of coated vesicles and early endosomal cisternae immediately below the basal bodies. Two rows of parasomal sacs border the closely packed peniculi and a third row separates the two peniculi. Four rows of parasomal sacs interdigitate with the 4 rows of more loosely associated cilia in the quadrulus. TEM taken on 1\/25\/74 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 19,500X. Bar = 0.2µm."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"oral apparatus","onto_id":"GO:0031912"},{"onto_name":"cilium","onto_id":"GO:0005929"},{"onto_name":"cell cortex","onto_id":"GO:0005938"},{"onto_name":"early endosome","onto_id":"GO:0005769"},{"onto_name":"coated pit","onto_id":"GO:0005905"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"9933","TECHNICALDETAILS":{"free_text":"Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39129) which is available for quantitative analysis. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":2992,"axis":"X"}},{"Space":{"Image_size":3446,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Paramecium caudatum","onto_id":"NCBITaxon:5885"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (University of Hawaii) (2011) CIL:36768, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil36768","DOI":"doi:10.7295\/W9CIL36768"}}}