{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1320379200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"38716.tif","Size":1800000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"38716.jpg","Size":447318,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"38716.zip","Size":1736124,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"Journal of Cell Biology","Href":"http:\/\/jcb.rupress.org\/content\/48\/3\/594.full.pdf+html?sid=6c2cff91-8475-4246-8ec9-caaf88a6a9f5"}],"PUBLISHED":["UW Goodenough and LA Staehelin (1971) Structural differentiation of stacked and unstacked chloroplast membranes. J Cell Biol 48:595-619."],"Contributors":["Ursula W. Goodenough","L. Andrew Staehelin"],"PUBMED":["4396088"]},"BIOLOGICALPROCESS":[{"onto_name":"photosynthesis","onto_id":"GO:0015979"},{"onto_name":"organelle organization","onto_id":"GO:0006996"}],"CELLLINE":[{"free_text":"ac-5"},{"free_text":"ac-31"}],"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Isolated chloroplast membranes of the unicellular photosynthetic alga Chlamydomonas reinhardtii were fixed, sectioned, and examined by transmission electron microscopy. Extensive regions of stacked thylakoids are seen in these wildtype cells. The section also includes the remnants of a mitochondrion (mi). See Fig 2 in Goodenough and Staehelin 1971 J Cell Biol 48:595-619."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"chloroplast","onto_id":"GO:0009507"},{"onto_name":"plastid","onto_id":"GO:0009536"},{"onto_name":"plastid thylakoid membrane","onto_id":"GO:0055035"}],"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"RELATIONTOINTACTCELL":[{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},{"onto_name":"isolated subcellular component","onto_id":"FBbi:00000579"}],"GROUP_ID":"11387","TECHNICALDETAILS":{"free_text":"Isolated chloroplast membranes were fixed with glutaraldehyde, post fixed with osmium tetroxide, dehydrated, embedded in epoxy resin, and thin sections cut, stained and examined with a Hitachi HU-11C TEM."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":1476,"axis":"X"}},{"Space":{"Image_size":1176,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Chlamydomonas reinhardtii","onto_id":"NCBITaxon:3055"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"tissue in plastic embedment","onto_id":"FBbi:00000016"}],"VISUALIZATIONMETHODS":[{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}]}},"Citation":{"Title":"Ursula W. Goodenough, L. Andrew Staehelin (2011) CIL:38716, Chlamydomonas reinhardtii. CIL. Dataset","ARK":"ark:\/b7295\/w9cil38716","DOI":"doi:10.7295\/W9CIL38716"}}}