{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1295758800},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"11989.zip","Size":9724032,"Mime_type":"application\/zip"},{"File_type":"Mp4","File_path":"11989_web.mp4","Size":6701893,"Mime_type":"video\/mp4"},{"File_type":"Jpeg","File_path":"11989.jpg","Size":27772,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["J Cell Bio 186:727-738, 2009"],"Contributors":["Ronald D. Vale","James A. Spudich","Eric R. Griffis"],"PUBMED":["19720876"]},"BIOLOGICALPROCESS":[{"onto_name":"mitotic anaphase","onto_id":"GO:0000090"},{"free_text":"kinesin-6 depletion"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"free_text":"S2"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"TIRF time-lapse imaging of myosin-GFP (left) and mCherry-tubulin (right) in an S2 cell depleted of kinesin-6 (Pav) by RNAi. Myosin-GFP localization to the equator and loss from the poles does not occur after RNAi kinesin-6; microtubules tend to be more homogeneous throughout the cortex and less bundled.\nVideo corresponds to Fig 3B and video 6 from J Cell Bio 186:727-738, 2009.  Note that the wild type control is available as another video in the series.  \n\nTotal internal reflection microscopy was performed using a microscope (Perfect-focus TE2000; Nikon) with a 100×, 1.45 NA objective (Nikon) and illumination from either a 488-nm argon laser (100 mW) or a 491-nm solid-state laser (100 mW) and a 561-nm solid-state laser (50 mW). For dual-color TIRF microscopy, we used a triplepass dichroic filter (z491\/561\/633rpc) and changed the emission filter (ET525\/50 or ET595\/50; both from Chroma Technology Corp.) with a filter wheel placed before the camera. In some cases, an excitation notch filter was used in the filter cube (NF01-405\/488\/561\/635; Semrock, Inc.). Images were typically captured every 2-3 s with a 50-200 ms exposure with an EM charge-coupled device camera (iXon; Andor Technology). The microscope was controlled and images were acquired using open source MicroManager software (http:\/\/www.micro-manager.org)."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"free_text":"myosin regulatory light chain"},{"onto_name":"spindle","onto_id":"GO:0005819"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"3733","DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"free_text":"TIRF"},"DIMENSION":[{"Space":{"Image_size":452,"Pixel_size":{"unit":"microns","value":0.096},"axis":"X"}},{"Space":{"Image_size":226,"Pixel_size":{"unit":"microns","value":0.096},"axis":"Y"}},{"Time":{"unit":"seconds","value":4}},{"Wavelength":{"unit":"nanometers","value":"488, 561"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Drosophila melanogaster","onto_id":"NCBITaxon:7227"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":[{"onto_name":"EGFP","onto_id":"FBbi:00000082"},{"onto_name":"mCherryFP","onto_id":"FBbi:00000525"}],"CELLTYPE":{"onto_name":"epithelial cell","onto_id":"CL:0000066"}}},"Citation":{"Title":"Ronald D. Vale, James A. Spudich, Eric R. Griffis (2011) CIL:11989, Drosophila melanogaster, epithelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil11989","DOI":"doi:10.7295\/W9CIL11989"}}}