{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1301284800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"13544.tif","Size":8500000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"13544.jpg","Size":94093,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"13544.zip","Size":8402032,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/2860\/"}],"PUBLISHED":["J Cell Biol. 189: 843-858. 2010."],"Contributors":["David J. Gill","Joanne Chia","Jamie Senewiratne","Frederic Bard"],"PUBMED":["PMID: 20498016"]},"BIOLOGICALPROCESS":[{"onto_name":"Golgi organization","onto_id":"GO:0007030"},{"onto_name":"protein O-linked glycosylation via serine","onto_id":"GO:0018242"},{"onto_name":"protein O-linked glycosylation via threonine","onto_id":"GO:0018243"}],"CELLLINE":{"onto_name":"HeLa","onto_id":"MCC:0000219"},"PARAMETERIMAGED":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"IMAGEDESCRIPTION":{"free_text":"Helix Pomatia Lectin (HPL) (green) staining at the Golgi (Giantin) (red) in unstimulated HeLa cells. HPL binds various glycans but the Tn antigen in particular. The Tn antigen refers to terminal α-linked N-acetyl galactosamine residues (GalNAc) linked to Ser or Thr residues. Image also includes anti-GalNacT1 staining (gray). HeLa cells were serum starved overnight in DME (noFBS). Cells were fixed for 10 min (4% paraformaldehyde) and permeabilized (0.2% Triton X-100). Primary antibody staining followed the manufacturer\u2019s instructions. Cells were subsequently stained for 15\u201330 min with secondary Alexa Fluor\u2013conjugated antibodies (Alexa 488 for anti-Giantin, Alexa 594 for anti GalNac-T1), Hoechst (blue) and Alexa 647-conjugated-HPL was added during secondary antibody incubations. Cells were mounted onto glass slides using FluorSave (Merck) and imaged at room temperature using an inverted FluoView confocal microscope (model IX81; Olympus) with fluorescence excitation at 488 nm, 561 nm, and 633 nm and either a 60x objective (U Plan Super Apochromatic [UPLSAPO]; NA 1.35) or 100x objective (UPLSAPO; NA 1.40) using Immersol oil. Microscope coupled with a CCD camera (model FVII). Images were acquired and processed using Olympus FV10-ASW software. Image corresponds to Fig 1A in J Cell Biol. 189: 843-858. 2010. Images in Fig 1 include CIL#s 13543, 13544, 13545, 13546."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"Golgi stack","onto_id":"GO:0005795"},{"onto_name":"integral to membrane","onto_id":"GO:0016021"},{"onto_name":"Golgi cisterna membrane","onto_id":"GO:0032580"},{"onto_name":"nucleus","onto_id":"GO:0005634"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":[{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"},{"onto_name":"compartmentalization of stain or label","onto_id":"FBbi:00000604"}],"GROUP_ID":"6490","MOLECULARFUNCTION":[{"onto_name":"manganese ion binding","onto_id":"GO:0030145"},{"onto_name":"polypeptide N-acetylgalactosaminyltransferase activity","onto_id":"GO:0004653"},{"onto_name":"N-acetylgalactosamine binding","onto_id":"GO:0046871"}],"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"single-spot confocal microscopy","onto_id":"FBbi:00000252"},"DIMENSION":[{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":0.207},"axis":"X"}},{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":0.207},"axis":"Y"}},{"Space":{"Image_size":1,"Pixel_size":{"unit":"microns","value":2.83},"axis":"Z"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"detergent permeabilized","onto_id":"FBbi:00000262"}],"VISUALIZATIONMETHODS":[{"onto_name":"Alexa Fluor 647","onto_id":"FBbi:00000447"},{"onto_name":"Alexa Fluor 594","onto_id":"FBbi:00000444"},{"onto_name":"Alexa Fluor 488","onto_id":"FBbi:00000440"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},{"onto_name":"Helix pomatia agglutinin","onto_id":"FBbi:00000429"},{"free_text":"Hoechst"}],"CELLTYPE":[{"onto_name":"epithelial cell","onto_id":"CL:0000066"},{"free_text":"cervical carcinoma"}]}},"Citation":{"Title":"David J. Gill, Joanne Chia, Jamie Senewiratne, Frederic Bard (2011) CIL:13544, Homo sapiens, epithelial cell, cervical carcinoma. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13544","DOI":"doi:10.7295\/W9CIL13544"}}}